基于金纳米粒子的无标记荧光 DNA 计算器用于灵敏检测 ATP。
A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP.
机构信息
State Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
出版信息
Molecules. 2018 Sep 29;23(10):2494. doi: 10.3390/molecules23102494.
Herein we described a deoxyribonucleic acid (DNA) calculator for sensitive detection of the determination of adenosine triphosphate (ATP) using gold nanoparticles (GNP) and PicoGreen fluorescence dye as signal transducer, and ATP and single-stranded DNA (DNA-M') as activators. The calculator-related performances including linearity, reaction time, logic gate, and selectivity were investigated, respectively. The results revealed that this oligonucleotide sensor was highly sensitive and selective. The detection range was 50⁻500 nmol/L (R² = 0.99391) and the detection limit was 46.5 nmol/L. The AND DNA calculator was successfully used for the ATP detection in human urine. Compared with other methods, this DNA calculator has the characteristics of being label-free, non-enzymic, simple, and highly sensitive.
在这里，我们描述了一种脱氧核糖核酸（DNA）计算器，用于使用金纳米粒子（GNP）和 PicoGreen 荧光染料作为信号转导物，灵敏检测三磷酸腺苷（ATP）的测定，并用 ATP 和单链 DNA（DNA-M'）作为激活剂。分别研究了计算器相关性能，包括线性度、反应时间、逻辑门和选择性。结果表明，这种寡核苷酸传感器具有高灵敏度和选择性。检测范围为 50⁻500nmol/L（R²=0.99391），检测限为 46.5nmol/L。AND DNA 计算器成功地用于人尿中 ATP 的检测。与其他方法相比，这种 DNA 计算器具有无标记、非酶、简单和高灵敏度的特点。
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