关于三个克氏锥虫种群两亲性蛋白质的研究。

A study on the amphiphilic proteins of three Trypanosoma cruzi populations.

作者信息

Giovanni-de-Simone S, Bonaldo M C, Pinho R T, Pontes-de-Carvalho L C, Galvão-Castro B, Goldenberg S

机构信息

Departamento de Imunologia (WHO Collaborating Centre on Immunology of Parasitic Diseases), Fundação Oswaldo Cruz, Rio de Janeiro, Brasil.

出版信息

Braz J Med Biol Res. 1988;21(3):435-43.

Abstract

Amphiphilic proteins were partially purified from culture-derived metacyclic trypomastigotes of the CL and Colombian strains and of the Dm 28c clone of T. cruzi by the use of Triton X-114. These proteins were subjected to one- and two-dimensional polyacrylamide electrophoresis in the presence of sodium dodecyl sulphate. 2. Relatively simple protein profiles with only 5 to 6 major bands were obtained. The CL and Colombian strains produced at least one additional major protein band (86 kDa) compared to the Dm 28c proteins. 3. Trypomastigote amphiphilic proteins displayed both electrophoretic mobilities and isoelectric points identical to those of two polypeptides precipitated by a rabbit antiserum which recognized metacyclic trypomastigote-specific surface antigens. 4. The partial purification of the T. cruzi amphiphilic proteins with Triton X-114 may provide a simple preparative step for the study of these differentiation-related polypeptides, as well as for the study of strain-specific (glyco)proteins and of their possible biological role.

摘要

通过使用Triton X-114，从克氏锥虫CL株和哥伦比亚株以及Dm 28c克隆的培养衍生的循环后期锥鞭毛体中部分纯化两亲性蛋白。这些蛋白在十二烷基硫酸钠存在下进行一维和二维聚丙烯酰胺电泳。2. 获得了相对简单的蛋白图谱，只有5至6条主要条带。与Dm 28c蛋白相比，CL株和哥伦比亚株产生了至少一条额外的主要蛋白条带（86 kDa）。3. 锥鞭毛体两亲性蛋白的电泳迁移率和等电点与兔抗血清沉淀的两种多肽相同，该抗血清识别循环后期锥鞭毛体特异性表面抗原。4. 用Triton X-114对克氏锥虫两亲性蛋白进行部分纯化，可能为研究这些与分化相关的多肽以及菌株特异性（糖）蛋白及其可能的生物学作用提供一个简单的制备步骤。