Hotson John R
Department of Neurology and Neurological Sciences, Stanford University, Stanford, California, United States of America; California Institute for Medical Research, San Jose, California, United States of America.
PLoS One. 2014 Mar 21;9(3):e92694. doi: 10.1371/journal.pone.0092694. eCollection 2014.
The peroneal nerve anatomy of the rabbit distal hindlimb is similar to humans, but reports of distal peroneal nerve conduction studies were not identified with a literature search. Distal sensorimotor recordings may be useful for studying rabbit models of length-dependent peripheral neuropathy. Surface electrodes were adhered to the dorsal rabbit foot overlying the extensor digitorum brevis muscle and the superficial peroneal nerve. The deep and superficial peroneal nerves were stimulated above the ankle and the common peroneal nerve was stimulated at the knee. The nerve conduction studies were repeated twice with a one-week intertest interval to determine measurement variability. Intravenous vincristine was used to produce a peripheral neuropathy. Repeat recordings measured the response to vincristine. A compound muscle action potential and a sensory nerve action potential were evoked in all rabbits. The compound muscle action potential mean amplitude was 0.29 mV (SD ± 0.12) and the fibula head to ankle mean motor conduction velocity was 46.5 m/s (SD ± 2.9). The sensory nerve action potential mean amplitude was 22.8 μV (SD ± 2.8) and the distal sensory conduction velocity was 38.8 m/s (SD ± 2.2). Sensorimotor latencies and velocities were least variable between two test sessions (coefficient of variation = 2.6-5.9%), sensory potential amplitudes were intermediate (coefficient of variation = 11.1%) and compound potential amplitudes were the most variable (coefficient of variation = 19.3%). Vincristine abolished compound muscle action potentials and reduced sensory nerve action potential amplitudes by 42-57% while having little effect on velocity. Rabbit distal hindlimb nerve conduction studies are feasible with surface recordings and stimulation. The evoked distal sensory potentials have amplitudes, configurations and recording techniques that are similar to humans and may be valuable for measuring large sensory fiber function in chronic models of peripheral neuropathies.
兔后肢远端的腓总神经解剖结构与人类相似，但通过文献检索未发现有关远端腓总神经传导研究的报告。远端感觉运动记录可能有助于研究长度依赖性周围神经病变的兔模型。将表面电极粘贴在兔足背覆盖趾短伸肌和腓浅神经的部位。在踝关节上方刺激腓深神经和腓浅神经，在膝关节处刺激腓总神经。神经传导研究以一周的测试间隔重复两次，以确定测量的可变性。静脉注射长春新碱以诱发周围神经病变。重复记录测量对长春新碱的反应。所有兔子均诱发出复合肌肉动作电位和感觉神经动作电位。复合肌肉动作电位平均幅度为0.29 mV（标准差±0.12），腓骨头至踝关节的平均运动传导速度为46.5 m/s（标准差±2.9）。感觉神经动作电位平均幅度为22.8 μV（标准差±2.8），远端感觉传导速度为38.8 m/s（标准差±2.2）。感觉运动潜伏期和速度在两次测试之间的变异性最小（变异系数=2.6 - 5.9%），感觉电位幅度居中（变异系数=11.1%），复合电位幅度变异性最大（变异系数=19.3%）。长春新碱消除了复合肌肉动作电位，并使感觉神经动作电位幅度降低了42 - 57%，而对速度影响很小。兔后肢远端神经传导研究通过表面记录和刺激是可行的。诱发出的远端感觉电位的幅度、形态和记录技术与人类相似，对于测量周围神经病变慢性模型中的大感觉纤维功能可能有价值。
