粒细胞集落刺激因子动员和未动员外周血中红系祖细胞的转录谱分析。

Transcriptional profiling of erythroid progenitors from G-CSF mobilized and nonmobilized peripheral blood.

作者信息

Cokic Vladan P, Diklic Milos, Suboticki Tijana, Beleslin-Cokic Bojana B, Markovic Dragana, Milenkovic Pavle, Jovcic Gordana

机构信息

Laboratory of Experimental Hematology, Institute for Medical Research, University of Belgrade, Belgrade, Serbia.

出版信息

J BUON. 2014 Jan-Mar;19(1):304-14.

PMID:24659680

Abstract

PURPOSE

The purpose of this study was to examine the gene expression profile of granulocyte colony stimulating factor (G-CSF)-mobilized peripheral blood (mPB)-derived progenitors, used in transplantation.

METHODS

We correlated gene expression patterns of highly enriched steady-state peripheral blood (PB)- and mPB-derived CD71+ cells by microarray and ingenuity pathway analyses, to identify the transcriptional program during in vitro erythroid differentiation.

RESULTS

The gene expression was more than doubled in mPB-derived (4180 genes) compared to PB-derived erythroid progenitors (1667 genes) while PB-and mPB-derived erythroid progenitors shared 1534 common genes. Comparative analysis of transcript levels showed differential expression of 54 genes between cultured erythroid progenitors of PB and mPB origin, where we identified common 13 downregulated and 30 upregulated genes. The most significant genes in mPB-derived erythroid progenitors were P4HB, DDIA3, ARPC2 and ATP5G3. Regarding G-CSF stimulation the G-CSF receptor CSF2RB (1.1-fold) was linked via STAT3 to erythroid-specific ALAS2 (2.9-fold) and GATA2 (1.3-fold) factors, all upregulated in mPB-derived erythroid progenitors, coupled to common upregulated NUDC gene involved in the proliferation of erythroid cells.

CONCLUSION

This report provides an extensive transcriptional profile of cultured erythroid progenitors and leads to a better understanding of diversity among the progenitor sources.

摘要

目的

本研究旨在检测用于移植的粒细胞集落刺激因子（G-CSF）动员的外周血（mPB）来源祖细胞的基因表达谱。

方法

我们通过微阵列和 Ingenuity 通路分析，关联了高度富集的稳态外周血（PB）和 mPB 来源的 CD71+细胞的基因表达模式，以确定体外红系分化过程中的转录程序。

结果

与 PB 来源的红系祖细胞（1667 个基因）相比，mPB 来源的红系祖细胞（4180 个基因）的基因表达增加了一倍多，而 PB 和 mPB 来源的红系祖细胞共有 1534 个共同基因。转录水平的比较分析显示，PB 和 mPB 来源的培养红系祖细胞之间有 54 个基因差异表达，其中我们确定了 13 个共同下调基因和 30 个共同上调基因。mPB 来源的红系祖细胞中最显著的基因是 P4HB、DDIA3、ARPC2 和 ATP5G3。关于 G-CSF 刺激，G-CSF 受体 CSF2RB（1.1 倍）通过 STAT3 与红系特异性 ALAS2（2.9 倍）和 GATA2（1.3 倍）因子相连，这些因子在 mPB 来源的红系祖细胞中均上调，与参与红系细胞增殖的共同上调的 NUDC 基因相关。