Suzuki T, Muroi K, Amemiya Y, Miura Y
Department of Medicine, Jichi Medical School, Japan.
Bone Marrow Transplant. 1998 Apr;21(8):751-7. doi: 10.1038/sj.bmt.1701162.
G-CSF administered to healthy volunteers at a dose of 3 microg/kg for 5 days mobilized colony-forming units granulocyte-macrophage (CFU-GM), burst-forming units-erythroid (BFU-E), and long-term culture-initiating cells (LTC-IC) to a maximal level on day 4 or 5. To determine the number of primitive hematopoietic progenitors in the peripheral blood, mononuclear cells (MNCs) or CD34+ cells were cultured in a long-term culture (LTC) system. We defined the colonies produced by cells during LTC at week 2 as differentiated progenitors and those at week 5 as primitive progenitors. G-CSF administered to healthy volunteers increased the number of differentiated progenitors from day 4 and primitive progenitors on days 3-5. Enriched CD34+ cells from healthy volunteers treated with G-CSF (PB-G) or without it (PB-SS), and patients treated with chemotherapy plus G-CSF (PB-CG) were subjected to LTC for 7 weeks. PB CD34+ cells from PB-G contained less primitive progenitors than those from PB-CG. Therefore, in healthy donors administered G-CSF at a dose of 3 microg/kg, the number of PB CD34+ cells should be harvested as much as possible to perform allogeneic peripheral blood stem cell transplantation (PBSCT).
以3微克/千克的剂量给健康志愿者注射粒细胞集落刺激因子（G-CSF），持续5天，可使集落形成单位粒细胞-巨噬细胞（CFU-GM）、爆式红细胞集落形成单位（BFU-E）和长期培养起始细胞（LTC-IC）在第4天或第5天达到最高水平。为了确定外周血中原始造血祖细胞的数量，将单核细胞（MNCs）或CD34+细胞在长期培养（LTC）系统中培养。我们将LTC第2周时细胞产生的集落定义为分化祖细胞，第5周时的集落定义为原始祖细胞。给健康志愿者注射G-CSF后，从第4天起分化祖细胞数量增加，在第3 - 5天原始祖细胞数量增加。对用G-CSF处理（PB-G）或未处理（PB-SS）的健康志愿者以及接受化疗加G-CSF治疗的患者（PB-CG）富集的CD34+细胞进行7周的LTC培养。来自PB-G的外周血CD34+细胞所含原始祖细胞比来自PB-CG的少。因此，对于以3微克/千克剂量接受G-CSF治疗的健康供体，应尽可能多地采集外周血CD34+细胞以进行异基因外周血干细胞移植（PBSCT）。
