Kurdistan Cellular & Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Kurdistan Cellular & Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran ; Kurdistan Liver & Digestive Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
J Oncol. 2014;2014:808012. doi: 10.1155/2014/808012. Epub 2014 Feb 10.
Introduction. Previous studies have shown that stromal-derived factor-1 (CXCL12) and its receptor, CXCR4, play a crucial role in metastasis of various tumors. Similarly, it has been cleared that CXCR4 is expressed on the cell surface of gastric cancers. However, nuclear expression of CXCR4 and its clinical importance have not been yet studied. Materials and Methods. Herein, we studied the expression of CXCR4 in gastric samples from patients with gastric adenocarcinoma as well as human gastric carcinoma cell line, AGS, by employing RT-PCR, immunohistochemistry, and flow cytometry techniques. Results. RT-PCR data showed that CXCR4 is highly expressed on AGS cells. This was confirmed by IHC and FACS as CXCR4 was detected on cell membrane, in cytoplasm, and in nucleus of AGS cells. Moreover, we found that both cytoplasmic and nuclear CXCR4 are strongly expressed in primary gastric cancer and the cytoplasmic pattern of CXCR4 tends to be associated with a shorter overall survival than nuclear staining. In conclusion, we present evidence for the first time that both cytoplasmic and nuclear expression of CXCR4 are detectable in gastric cancer tissues. However, the role of both cytoplasmic and nuclear CXCR4 needs to be further elucidated.
简介。先前的研究表明，基质衍生因子-1（CXCL12）及其受体 CXCR4 在各种肿瘤的转移中起着至关重要的作用。同样，已经明确 CXCR4 表达在胃癌细胞的细胞表面。然而，CXCR4 的核表达及其临床意义尚未得到研究。
材料和方法。在此，我们通过 RT-PCR、免疫组织化学和流式细胞术技术研究了 CXCR4 在患有胃腺癌的患者的胃样本以及人胃癌细胞系 AGS 中的表达。
结果。RT-PCR 数据显示，CXCR4 在 AGS 细胞中高表达。免疫组织化学和流式细胞术证实了这一点，因为在 AGS 细胞的细胞膜、细胞质和核内都检测到了 CXCR4。此外，我们发现原发性胃癌中细胞质和核内的 CXCR4 均强烈表达，且 CXCR4 的细胞质模式与总生存期较短相关。
结论，我们首次证明在胃癌组织中可检测到 CXCR4 的细胞质和核表达。然而，细胞质和核内 CXCR4 的作用仍需要进一步阐明。
