Ito K, Ishii A, Yamashita N, Miyamoto T, Watanabe N
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan.
Int Arch Allergy Appl Immunol. 1988;87(4):424-9. doi: 10.1159/000234713.
Guinea pig IgE antibodies to ovalbumin or mite were measured by enzyme-linked immunosorbent assay (ELISA), and the levels of IgE antibodies to mite were compared with titers of passive cutaneous anaphylaxis (PCA). The results of ELISA were highly reproducible when the assays were done in the same microtiter plate and on the same day. The levels of IgE antibodies to mite determined by ELISA correlated well with the PCA titers (n = 29, r = 0.70, p less than 0.001). The measurement of IgE antibodies by ELISA saved much time in comparison with PCA which needs at least 7 days for getting results.
通过酶联免疫吸附测定法（ELISA）检测豚鼠针对卵清蛋白或螨虫的IgE抗体，并将螨虫IgE抗体水平与被动皮肤过敏反应（PCA）滴度进行比较。当在同一天在同一微孔板中进行检测时，ELISA结果具有高度可重复性。ELISA测定的螨虫IgE抗体水平与PCA滴度相关性良好（n = 29，r = 0.70，p小于0.001）。与PCA相比，ELISA检测IgE抗体节省了大量时间，PCA至少需要7天才能得到结果。
