Ghazizadeh M, Kagawa S, Oguro T, Takigawa H, Sasaki Y, Aihara K
Division of Immunohistochemistry, Nippon Medical School, Tokyo, Japan.
J Cancer Res Clin Oncol. 1989;115(1):84-8. doi: 10.1007/BF00391605.
Thirty-seven specimens of benign and malignant prostatic tumors were studied for the localization of tissue polypeptide antigen (TPA) by an avidin-biotin-peroxidase complex technique. In addition, 23 metastases of prostatic carcinoma in other organs and 12 nonepithelial tumors of prostate also were studied. All benign and malignant tumors of epithelial origin, including their metastasis, stained positively. Nonepithelial tumors were uniformly negative. In the metastatic lesions, small foci of tumor cells and even single tumor cells could be identified by TPA staining. Immunohistochemical localization of TPA appeared to be a useful tool for assessing the micrometastases of prostatic carcinoma in other organs, especially lymph nodes, or elucidating the epithelial origin of an otherwise undifferentiated prostatic cancer.
采用抗生物素蛋白-生物素-过氧化物酶复合物技术，对37例前列腺良恶性肿瘤标本进行了组织多肽抗原（TPA）定位研究。此外，还研究了23例前列腺癌在其他器官的转移灶以及12例前列腺非上皮性肿瘤。所有上皮源性良恶性肿瘤，包括其转移灶，均呈阳性染色。非上皮性肿瘤均为阴性。在转移病灶中，TPA染色可识别出小灶肿瘤细胞甚至单个肿瘤细胞。TPA的免疫组化定位似乎是评估前列腺癌在其他器官尤其是淋巴结中的微转移，或阐明未分化前列腺癌上皮起源的有用工具。
