Menendez-Botet C J, Oettgen H F, Pinsky C M, Schwartz M K
Clin Chem. 1978 Jun;24(6):868-72.
We evaluated assays of Tissue Polypeptide Antigen in serum and urine, as an index to the presence of cancer. In the assay, serum, which is first absorbed with human albumin-labeled sheep erythrocytes, or untreated urine (diluted with an equal volume of TPA-free serum) is incubated with antibody specific to Tissue Polypeptide Antigen and then reacted with sheep erythrocytes labeled with Tissue Polypeptide Antigen. We found an increased concentration of Tissue Polypeptide Antigen in the serum of 378 of 513 (74%) and in the urine of 49 of 77 (64%) patients with cancer, as compared with 40/112 (36%) and 7/29 (24%), respectively, for individuals with benign neoplasms. Normal individuals were defined as those with less than 0.09 unit of the antigen per milliliter of specimen. Concentrations exceeding this were found in 2/67 (3%) sera and 6/56 (11%) urines from supposedly normal persons. Tissue Polypeptide Antigen was found in above-normal concentrations in patients with cancer, regardless of neoplasm type and extension, with a higher proportion of abnormal values in patients with distal metastases.
我们评估了血清和尿液中组织多肽抗原检测作为癌症存在指标的情况。在该检测中，首先用人类白蛋白标记的绵羊红细胞吸附的血清或未处理的尿液（用等体积不含组织多肽抗原的血清稀释）与组织多肽抗原特异性抗体孵育，然后与用组织多肽抗原标记的绵羊红细胞反应。我们发现，513例癌症患者中有378例（74%）血清中组织多肽抗原浓度升高，77例中有49例（64%）尿液中该抗原浓度升高，相比之下，良性肿瘤患者中分别为40/112（36%）和7/29（24%）。正常个体定义为每毫升标本中抗原含量低于0.09单位的个体。在假定正常的人的2/67（3%）份血清和6/56（11%）份尿液中发现浓度超过此值。无论肿瘤类型和分期如何，癌症患者中均发现组织多肽抗原浓度高于正常，远端转移患者中异常值比例更高。
