Kennedy A, Foulis A K
Department of Pathology, Royal Infirmary, Glasgow.
J Clin Pathol. 1989 Jan;42(1):101-5. doi: 10.1136/jcp.42.1.101.
The quality of microscopic image of cryostat sections that had been subjected to microwave assisted fixation was compared with that resulting from conventional air drying of the sections. The role of microwaves in producing rapid special stains on cryostat sections was also assessed. Methods used permitted stains such as periodic acid Schiff, alcian blue, Gordon and Sweets's reticulin, Masson Fontana, Elastica, Prussian blue and Van Gieson to be performed within three minutes of cutting a cryostat section. The cytological detail of nuclei was much clearer using the microwave technique, allowing more accurate determination of cell type. The microwave oven seems to have major potential in improving the diagnostic accuracy of surgical frozen sections.
将经过微波辅助固定的冷冻切片的显微图像质量与传统空气干燥切片的显微图像质量进行了比较。还评估了微波在冷冻切片上快速进行特殊染色的作用。所采用的方法使诸如过碘酸希夫染色、阿尔辛蓝染色、戈登和斯威茨网状纤维染色、马森·丰塔纳染色、弹性纤维染色、普鲁士蓝染色和范吉森染色等染色能够在切割冷冻切片后的三分钟内完成。使用微波技术时，细胞核的细胞学细节要清晰得多，从而能够更准确地确定细胞类型。微波炉在提高手术冷冻切片的诊断准确性方面似乎具有巨大潜力。
