Central Aquaculture Genetics Laboratory, Rajiv Gandhi Centre for Aquaculture, 3/197, Poompuhar Road, Karaimedu Village, Sattanathapuram P.O., SirkaliTamil Nadu, Nagapattinam, 609 109, India.
Aquaculture Department, Southeast Asian Fisheries Development Center, Iloilo, Philippines.
Biochem Genet. 2021 Aug;59(4):856-869. doi: 10.1007/s10528-021-10032-3. Epub 2021 Feb 5.
Misleading identification and subsequent publications on biological, molecular, and aquaculture data of mangrove mud crab (genus Scylla de Hann 1833) is a major concern in many countries. In this study, multiple molecular markers were used for genetic identification of all four known mud crab species under genus Scylla collected from India, Philippines, Myanmar, Malaysia and Indonesia. Internal Transcribed Spacer (ITS-1), Polymerase chain reaction (PCR)-Restriction Fragment Length Polymorphism (PCR-RFLP) and PCR-based species-specific markers were used to resolve taxonomic ambiguity. PCR-RFLP techniques using NlaIV and BsaJI restriction endonucleases were efficient to differentiate four different mud crab species under genus Scylla with specific fragment profile. The results also justified the use of ITS-1 and PCR-based species-specific markers to identify mud crab species available in many countries quite rapidly and effectively. Several new molecular markers generated during the study are reported here to resolve the taxonomic ambiguity of Scylla species and the results reconfirmed that India is only having two commonly available mud crab species which was reported by the authors earlier.
在许多国家，红树林招潮蟹（Scylla de Hann 1833 属）的生物、分子和水产养殖数据的错误鉴定和后续发表是一个主要问题。在这项研究中，使用了多种分子标记物来鉴定从印度、菲律宾、缅甸、马来西亚和印度尼西亚收集的 Scylla 属的所有四种已知招潮蟹。使用内部转录间隔区（ITS-1）、聚合酶链反应（PCR）-限制性片段长度多态性（PCR-RFLP）和基于 PCR 的种特异性标记物来解决分类学上的模糊性。使用 NlaIV 和 BsaJI 限制性内切酶的 PCR-RFLP 技术能够有效地区分 Scylla 属的四种不同的招潮蟹，具有特定的片段图谱。研究结果还证明，使用 ITS-1 和基于 PCR 的种特异性标记物可以快速有效地鉴定许多国家可用的招潮蟹物种。本研究中还生成了一些新的分子标记物，以解决 Scylla 物种的分类学模糊性，结果再次证实，印度只有作者早些时候报道的两种常见的招潮蟹物种。
