Chen Xiao, Ai Weiming, Pan Lianghao, Shi Xiaofang
a Guangxi Key Lab for Mangrove Conservation and Utilization , Guangxi Mangrove Research Center, Guangxi Academy of Sciences , Beihai , PR China and.
b Department of Marine Biotechnology , School of Life Science, Wenzhou Medical University , Wenzhou , PR China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):425-6. doi: 10.3109/19401736.2014.898288. Epub 2014 Mar 24.
The whale shark Rhincodon typus (Pisces: Chondrichthyes, Orectolobiformes, Rhincodontidae) is the largest living fish on Earth. In this study, we presented its complete mitogenome. It is 16,928 bp in length, contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and one control region with the typical gene order and transcriptional direction in the vertebrates. Overall base composition of the R. typus mitogenome is 33.5% A, 24.3% C, 12.8% G and 29.5% T. Two start codon (GTG and ATG) and two stop codon patterns (TAG and TAA/T) were found in protein-coding genes. The tRNA-Ser2 could not be folded into the typical cloverleaf secondary structure because of the replacement of its dihydrouridine arm by a simple loop. A termination associated sequences (TAS) and three conserved sequence blocks (CSB1-3) were identified in the control region.
鲸鲨(学名:Rhincodon typus,纲:硬骨鱼纲,目:须鲨目,科:鲸鲨科)是地球上现存最大的鱼类。在本研究中,我们展示了其完整的线粒体基因组。它全长16928碱基对,包含13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因以及一个控制区,其基因顺序和转录方向在脊椎动物中具有典型性。鲸鲨线粒体基因组的总体碱基组成为:腺嘌呤(A)占33.5%、胞嘧啶(C)占24.3%、鸟嘌呤(G)占12.8%、胸腺嘧啶(T)占29.5%。在蛋白质编码基因中发现了两种起始密码子(GTG和ATG)以及两种终止密码子模式(TAG和TAA/T)。由于其二氢尿嘧啶臂被一个简单环取代,转运RNA - Ser2无法折叠成典型的三叶草二级结构。在控制区鉴定出一个终止相关序列(TAS)和三个保守序列块(CSB1 - 3)。