Rojas-Cano M L, Ruiz-Guerrero V, Lara L, Nieto R, Aguilera J F
Department of Physiology and Biochemistry of Animal Nutrition, Animal Nutrition Institute (INAN), Estación Experimental del Zaidín (CSIC), Camino del Jueves s/n, 18100 Armilla, Granada, Spain.
J Anim Sci. 2014 Apr;92(4):1595-603. doi: 10.2527/jas.2013-6947. Epub 2014 Mar 18.
The effects of dietary protein content on the rates of gain and protein deposition were studied in Iberian (IB) × Duroc (DU) 50:50 barrows at 2 stages of growth [10.6 ± 0.2 (n = 28) and 60.0 ± 0.4 (n = 24) kg initial BW]. Two feeding, digestibility, and N-balance trials were performed. At each stage of growth, they were allocated in individual pens and given restrictedly (at 0.9 × ad libitum intake) one of 4 pelleted diets of similar energy concentration (13.8 to 14.5 MJ ME/kg DM), formulated to provide 4 different (ideal) CP contents (236, 223, 208, and 184 g CP/kg DM in the first trial, and 204, 180, 143, and 114 g CP/kg DM in the second trial). Feed allowance was offered in 2 daily equal meals. The average concentration of Lys was 6.59 ± 0.13 g /100 g CP for all diets. Whatever the stage of growth, average daily BW gain and gain to feed ratio were unchanged by increases in dietary CP content (477 ± 7 and 1,088 ± 20 g, and 0.475 ± 0.027 and 0.340 ± 0.113, respectively, in the first and second trial). In pigs growing from 10 to 27 kg BW, the average rate of N retention increased linearly (P < 0.01) on increasing the protein content in the diet up to a break point, so a linear-plateau dose response was observed. Pigs fed diets providing 208 to 236 g/kg DM did not differ in rate of protein deposition (PD). A maximum value of 87 (13.93 g N retained × 6.25) g PD/d was obtained when the diet supplied at least 208 g CP/kg DM. The broken-line regression analysis estimated dietary CP requirements at 211 g ideal CP (15.2 g total Lys)/kg DM. In the fattening pigs, there was a quadratic response (P < 0.01) in the rate of N retention as dietary CP content increased. Maximum N retention (18.7 g/d) was estimated from the first derivative of the function that relates the observed N retained (g/d) and dietary CP content (g/kg DM). This maximum value would be obtained by feeding a diet containing 185 g ideal CP (13.3 g total Lys)/kg DM and represents the maximum capacity of the IB × DU 50:50 pigs for protein accretion (i.e., 117 g PD/d). Consequently, cross breeding of IB dams with Duroc sires alters the metabolic response to dietary CP supply by increasing the potential of the crossbred to accrete protein and by modifying the pattern of PD response with BW.
研究了日粮蛋白质含量对伊比利亚(IB)×杜洛克(DU)二元杂交(50:50)公猪两个生长阶段[初始体重分别为10.6±0.2(n = 28)和60.0±0.4(n = 24)kg]生长速度和蛋白质沉积率的影响。进行了两项饲养、消化率和氮平衡试验。在每个生长阶段,将猪分配到个体栏中,并限量(按自由采食量的0.9倍)饲喂4种能量浓度相似(13.8至14.5 MJ ME/kg DM)的颗粒饲料中的一种,这4种饲料的粗蛋白(CP)含量不同(理想水平)(第一次试验中分别为236、223、208和184 g CP/kg DM,第二次试验中分别为204、180、143和114 g CP/kg DM)。每天分两次等量投喂饲料。所有日粮的赖氨酸平均浓度为6.59±0.13 g /100 g CP。无论处于哪个生长阶段,日粮CP含量增加时,平均日增重和料重比均无变化(第一次试验分别为477±7和1,088±20 g,第二次试验分别为0.475±0.027和0.340±0.113)。在体重从10 kg增长到27 kg的猪中,日粮蛋白质含量增加至转折点前,氮保留率呈线性增加(P < 0.01),因此观察到线性-平台剂量反应。饲喂CP含量为208至236 g/kg DM日粮的猪,蛋白质沉积率(PD)无差异。当日粮CP含量至少为208 g/kg DM时,PD的最大值为87(13.93 g氮保留×6.25)g/d。折线回归分析估计日粮CP需求量为211 g理想CP(15.2 g总赖氨酸)/kg DM。在育肥猪中,随着日粮CP含量增加,氮保留率呈二次反应(P < 0.01)。根据观察到的氮保留量(g/d)与日粮CP含量(g/kg DM)之间的函数一阶导数估计最大氮保留量(18.7 g/d)。通过饲喂含185 g理想CP(13.3 g总赖氨酸)/kg DM的日粮可获得该最大值,这代表了IB×DU 50:50二元杂交猪的最大蛋白质沉积能力(即117 g PD/d)。因此,用杜洛克公猪与伊比利亚母猪杂交,可改变杂交猪对日粮CP供应的代谢反应,增加其蛋白质沉积潜力,并改变PD反应随体重变化的模式。