以喷雾干燥血细胞或玉米蛋白粉为蛋白质来源的日粮中，8至25千克猪标准化回肠可消化异亮氨酸与赖氨酸最佳比例的估计。
Estimation of the optimum ratio of standardized ileal digestible isoleucine to lysine for eight- to twenty-five-kilogram pigs in diets containing spray-dried blood cells or corn gluten feed as a protein source.
作者信息
Wiltafsky M K, Bartelt J, Relandeau C, Roth F X
机构信息
Lehrstuhl für Tierernährung, Technische Universität München, Department für Tierwissenschaften, Hochfeldweg 6, Freising, Germany.
出版信息
J Anim Sci. 2009 Aug;87(8):2554-64. doi: 10.2527/jas.2008-1320. Epub 2009 Mar 27.
Two growth assays and 1 N balance trial were conducted to determine the standardized ileal digestible (SID) Ile:Lys ratio in 8- to 25-kg pigs using spray-dried blood cells or corn gluten feed as a protein source. In Exp. 1, 48 individually penned pigs (initial BW = 7.7 kg) were used in a 6-point SID Ile titration study (analyzed SID Ile of 0.36, 0.43, 0.50, 0.57, 0.64, and 0.72%) by addition of graded levels of L-Ile. The basal diet contained 1.00% SID Lys, 18.4% CP, and 13.6 MJ of ME/kg. Diets were based on wheat, barley, corn, and 7.5% spray-dried blood cells as a protein source. Dietary SID Leu and Val levels were 1.61 and 1.02%, respectively. For the 35-d period, ADG, ADFI, and G:F increased linearly (P < 0.01) and quadratically (P < 0.04) with increasing SID Ile:Lys. Estimates of optimal SID Ile:Lys ratios were 59% for ADG and ADFI. In Exp. 2, 24 N balances were conducted using the Exp. 1 diets (12 pigs; individually penned; average BW = 11.5 kg). Pigs were fed 3 times daily with an amount equal to 1.0 MJ of ME/kg of BW(0.75). Preparation and collection periods (7 d each) were repeated after rearranging the animals to treatments. Increasing the dietary SID Ile:Lys ratio increased N retention linearly (P < 0.01), and N utilization linearly (P < 0.01) and quadratically (P < 0.01). An optimal SID Ile:Lys ratio of 54% was estimated for N retention. In Exp. 3, 48 individually penned pigs (initial BW = 8.0 kg) were fed grain-based diets in a 6-point SID Ile titration (analyzed SID Ile of 0.35, 0.41, 0.49, 0.56, 0.62, and 0.69%). Dietary SID Ile was increased by graded addition of L-Ile. The basal diet contained 0.97% SID Lys, 16.8% CP, and 13.6 MJ of ME/kg. In contrast to Exp. 1 and 2, spray-dried blood cells were excluded and corn gluten feed was used as a protein source. Dietary SID Leu and Val were set to 1.05 and 0.66%. For the 42-d period, ADG, ADFI, and G:F increased linearly (P < 0.01) and quadratically (P < 0.01) with increasing SID Ile:Lys. Estimated optimal SID Ile:Lys ratios were 54, 54, and 49 for ADG, ADFI, and G:F, respectively. These experiments suggest that the optimal SID Ile:Lys ratio depends on diet composition. In Exp. 1, AA imbalances because of increased Leu contents may have led to increased Ile nutritional needs. For ADG and ADFI, an optimum SID Ile:Lys ratio of 54% was estimated for 8- to 25-kg pigs in diets without Leu excess.
进行了两项生长试验和一项氮平衡试验，以确定8至25千克猪使用喷雾干燥血细胞或玉米蛋白粉作为蛋白质来源时的标准回肠可消化（SID）异亮氨酸：赖氨酸比例。在试验1中，48头单独饲养的猪（初始体重=7.7千克）用于一项6点SID异亮氨酸滴定研究（分析的SID异亮氨酸水平为0.36%、0.43%、0.50%、0.57%、0.64%和0.72%），通过添加分级水平的L-异亮氨酸。基础日粮含有1.00%的SID赖氨酸、18.4%的粗蛋白和13.6兆焦代谢能/千克。日粮以小麦、大麦、玉米为基础，并使用7.5%的喷雾干燥血细胞作为蛋白质来源。日粮中的SID亮氨酸和缬氨酸水平分别为1.61%和1.02%。在35天的试验期内，随着SID异亮氨酸：赖氨酸比例的增加，平均日增重（ADG）、平均日采食量（ADFI）和料重比（G:F）呈线性（P<0.01）和二次曲线（P<0.04）增加。ADG和ADFI的最佳SID异亮氨酸：赖氨酸比例估计为59%。在试验2中，使用试验1的日粮进行了24次氮平衡试验（12头猪；单独饲养；平均体重=11.5千克）。猪每天饲喂3次，饲喂量相当于每千克体重代谢能1.0兆焦（0.75）。在将动物重新安排处理后，重复准备期和收集期（各7天）。提高日粮SID异亮氨酸：赖氨酸比例可使氮保留呈线性增加（P<0.01），氮利用率呈线性（P<0.01）和二次曲线（P<0.01）增加。氮保留的最佳SID异亮氨酸：赖氨酸比例估计为54%。在试验3中，48头单独饲养的猪（初始体重=8.0千克）饲喂以谷物为基础的日粮，进行6点SID异亮氨酸滴定（分析的SID异亮氨酸水平为0.35%、0.41%、0.49%、0.56%、0.62%和0.69%）。通过分级添加L-异亮氨酸来提高日粮中的SID异亮氨酸水平。基础日粮含有0.97%的SID赖氨酸、16.8%的粗蛋白和13.6兆焦代谢能/千克。与试验1和2不同，排除了喷雾干燥血细胞，使用玉米蛋白粉作为蛋白质来源。日粮中的SID亮氨酸和缬氨酸设定为1.05%和0.66%。在42天的试验期内，随着SID异亮氨酸：赖氨酸比例的增加，ADG、ADFI和G:F呈线性（P<0.01）和二次曲线（P<0.01）增加。ADG、ADFI和G:F的最佳SID异亮氨酸：赖氨酸比例估计分别为54%、54%和49%。这些试验表明，最佳SID异亮氨酸：赖氨酸比例取决于日粮组成。在试验1中，由于亮氨酸含量增加导致的氨基酸失衡可能导致异亮氨酸营养需求增加。对于ADG和ADFI，估计8至25千克猪在无亮氨酸过量的日粮中的最佳SID异亮氨酸：赖氨酸比例为54%。
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