真核 mRNA 5’-帽结构的生物正交定点标记。
Bioorthogonal site-specific labeling of the 5'-cap structure in eukaryotic mRNAs.
机构信息
Institute of Biochemistry, University of Muenster, Wilhelm-Klemm-Str. 2, D-48149 Münster, Germany.
出版信息
Chem Commun (Camb). 2014 May 4;50(34):4478-81. doi: 10.1039/c4cc01549e. Epub 2014 Mar 24.
PMID:24664183
Abstract
We present a chemo-enzymatic approach for site-specific labeling of 5'-capped RNAs based on bioorthogonal chemistry. A trimethylguanosine synthase was engineered to transfer a terminal azido moiety to the 5'-cap which could be further modified using strain-promoted azide-alkyne cycloaddition (SPAAC).
摘要
我们提出了一种基于生物正交化学的用于 5' 帽状 RNA 定点标记的化学酶法。通过工程改造三甲基鸟苷合酶将末端叠氮基团转移到 5' 帽上，然后进一步使用应变促进的叠氮-炔环加成（SPAAC）进行修饰。
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