Department of Pharmaceutical Sciences, University of Kentucky College of Pharmacy, Lexington, KY 40536, United States.
Department of Pharmaceutical Sciences, University of Kentucky College of Pharmacy, Lexington, KY 40536, United States; Center for Pharmaceutical Research and Innovation, University of Kentucky College of Pharmacy, Lexington, KY 40536, United States.
Curr Opin Biotechnol. 2021 Jun;69:290-298. doi: 10.1016/j.copbio.2021.02.010. Epub 2021 Apr 24.
Transferases have emerged as among the best catalysts for enzyme-mediated bioorthogonal functional group installation to advance innovative in vitro, cell-based and in vivo chemical biology applications. This review introduces the key considerations for selecting enzyme catalysts and chemoselective reactions most amenable to bioorthogonal platform development and highlights relevant key technology development and applications for one ubiquitous transferase subclass - methyltransferases (MTs). Within this context, recent advances in MT-enabled bioorthogonal labeling/conjugation relevant to DNA, RNA, protein, and natural products (i.e. complex small molecule metabolites) are highlighted.
转移酶已成为酶介导的生物正交官能团安装的最佳催化剂之一，可推动创新的体外、基于细胞和体内化学生物学应用。本综述介绍了选择酶催化剂和最适合生物正交平台开发的化学选择性反应的关键考虑因素，并重点介绍了一种普遍的转移酶亚类——甲基转移酶 (MTs) 的相关关键技术发展和应用。在此背景下，重点介绍了与 DNA、RNA、蛋白质和天然产物（即复杂的小分子代谢物）相关的 MT 介导的生物正交标记/缀合的最新进展。
