Saeidifar Maryam, Mansouri-Torshizi Hassan, Palizdar Y, Eslami-Moghaddam M, Divsalar Adeleh, Saboury Ali Akbar
Acta Chim Slov. 2014;61(1):126-36.
A new anionic 8-hydroxyquinolinatopalladate(II) complex with malonate has been synthesized and characterized by elemental analysis, conductivity, FT-IR, UV-Vis and 1H NMR techniques to enhance the development of potential anticancer agents. Cytotoxicity was determined against the human leukemia cells, molt, by MTT assay. The novel antitumor Pd(II) complex was evaluated for its binding to calf thymus DNA (ctDNA) in physiological buffer (pH 7.0) by using absorption spectroscopy, fluorescence titration spectra, ethidium bromide displacement and gel chromatography studies. The results obtained from these analyses indicated that the water-soluble complex can bind to DNA cooperatively through a static quenching procedure at low concentrations. Thermodynamic parameters obtained from fluorescence experiments at different temperatures revealed the hydrogen binding and van der Waals force in the binding process which was supported by Scatchard's plots.
合成了一种新的含丙二酸根的阴离子型8-羟基喹啉钯（II）配合物，并通过元素分析、电导率、傅里叶变换红外光谱（FT-IR）、紫外可见光谱（UV-Vis）和核磁共振氢谱（1H NMR）技术对其进行了表征，以促进潜在抗癌药物的开发。通过MTT法测定了该配合物对人白血病细胞系molt的细胞毒性。利用吸收光谱、荧光滴定光谱、溴化乙锭置换和凝胶色谱研究，评估了这种新型抗肿瘤钯（II）配合物在生理缓冲液（pH 7.0）中与小牛胸腺DNA（ctDNA）的结合情况。这些分析结果表明，该水溶性配合物在低浓度下可通过静态猝灭过程与DNA协同结合。在不同温度下进行荧光实验得到的热力学参数揭示了结合过程中的氢键和范德华力，这得到了Scatchard图的支持。
