Riegman P H, Vlietstra R J, van der Korput J A, Romijn J C, Trapman J
Department of Pathology, Erasmus University, Rotterdam, The Netherlands.
Biochem Biophys Res Commun. 1989 Feb 28;159(1):95-102. doi: 10.1016/0006-291x(89)92409-1.
Using Prostate-specific Antigen cDNA fragments as hybridization probes a clone containing the information for the gene encoding Prostate-specific Antigen was isolated form a human genomic DNA library. The complete gene (about 6 kb) was sequenced and shown to be composed of four introns and five exons. Two major transcription initiation sites were found. The sequence of the promoter region revealed the presence of various well known transcription regulatory elements including a TATA box. A high percentage of homology was found between the Prostate-specific Antigen gene and the hGK-1 gene (82%). This homology extended into the promoter region. Two previously described variant Prostate-specific Antigen cDNAs can now be explained by intron retention and alternative splicing of the primary transcript.
利用前列腺特异性抗原cDNA片段作为杂交探针，从人基因组DNA文库中分离出一个包含编码前列腺特异性抗原基因信息的克隆。对完整基因（约6kb）进行测序，结果显示其由四个内含子和五个外显子组成。发现了两个主要的转录起始位点。启动子区域的序列显示存在各种众所周知的转录调控元件，包括一个TATA盒。前列腺特异性抗原基因与hGK-1基因之间存在高度同源性（82%）。这种同源性延伸至启动子区域。先前描述的两种前列腺特异性抗原cDNA变体现在可以通过初级转录本的内含子保留和可变剪接来解释。
