Gomez-Alvarez Vicente
Office of Research and Development, U.S. Environmental Protection Agency, 26 W. Martin Luther King Drive, Cincinnati, OH, 45268, USA,
Methods Mol Biol. 2014;1147:323-40. doi: 10.1007/978-1-4939-0467-9_23.
Advances in high-throughput next-generation sequencing (NGS) technology for direct sequencing of environmental DNA (i.e., shotgun metagenomics) are transforming the field of microbiology. NGS technologies are now regularly being applied in comparative metagenomic studies, which provide the data for functional annotations, taxonomic comparisons, community profile, and metabolic reconstructions. For example, comparative metagenomic analysis of corroded pipes unveiled novel insights on the bacterial populations associated with the sulfur and nitrogen cycle, which may be directly or indirectly implicated in concrete wastewater pipe corrosion. The objective of this chapter is to describe the steps involved in the taxonomic and functional analysis of metagenome datasets from biofilm involved in microbial-induced concrete corrosion (MICC).
用于环境DNA直接测序的高通量下一代测序（NGS）技术（即鸟枪法宏基因组学）的进展正在改变微生物学领域。NGS技术现在经常应用于比较宏基因组研究，这些研究提供了功能注释、分类比较、群落概况和代谢重建的数据。例如，对腐蚀管道的比较宏基因组分析揭示了与硫和氮循环相关的细菌种群的新见解，这些细菌种群可能直接或间接与混凝土污水管道腐蚀有关。本章的目的是描述对参与微生物诱导混凝土腐蚀（MICC）的生物膜宏基因组数据集进行分类和功能分析所涉及的步骤。
