在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离后，对硝酸纤维素膜上蛋白质金染色相关假象的展示与消除。
Demonstration and elimination of artifacts associated with gold staining of proteins on nitrocellulose membranes after separation by sodium dodecyl sulphate-polyacrylamide gel electrophoresis.
作者信息
Bath M L
机构信息
Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra.
出版信息
Electrophoresis. 1988 Mar;9(3):148. doi: 10.1002/elps.1150090309.
PMID:2466652
Abstract
Artifacts associated with gold staining of polypeptides separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred to nitrocellulose are eliminated by alkylation of reduced samples or by deletion of the reducing agent.
摘要
通过对还原后的样品进行烷基化处理或去除还原剂，可消除与十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离并转移至硝酸纤维素膜上的多肽的金染色相关的假象。
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