Kovarík A, Hlubinová K, Vrbenská A, Prachar J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Folia Biol (Praha). 1987;33(4):253-7.
A simple method of revealing proteins on nitrocellulose blots is described. It is based on high affinity of colloidal silver for proteins. The sensitivity is about the same as that of colloidal gold and it appears to be about 20 times higher than the presently used staining methods. The whole procedure is very simple and does not take more than 30 min.
本文描述了一种在硝酸纤维素膜印迹上显示蛋白质的简单方法。该方法基于胶体银对蛋白质的高亲和力。其灵敏度与胶体金大致相同,似乎比目前使用的染色方法高约20倍。整个过程非常简单,耗时不超过30分钟。