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一种改进的用于硝酸纤维素膜上蛋白质印迹的胶体银染色方法。

An improved colloidal silver staining method of protein blots on nitrocellulose membranes.

作者信息

Kovarík A, Hlubinová K, Vrbenská A, Prachar J

机构信息

Cancer Research Institute, Slovak Academy of Sciences, Bratislava.

出版信息

Folia Biol (Praha). 1987;33(4):253-7.

PMID:2444470
Abstract

A simple method of revealing proteins on nitrocellulose blots is described. It is based on high affinity of colloidal silver for proteins. The sensitivity is about the same as that of colloidal gold and it appears to be about 20 times higher than the presently used staining methods. The whole procedure is very simple and does not take more than 30 min.

摘要

本文描述了一种在硝酸纤维素膜印迹上显示蛋白质的简单方法。该方法基于胶体银对蛋白质的高亲和力。其灵敏度与胶体金大致相同,似乎比目前使用的染色方法高约20倍。整个过程非常简单,耗时不超过30分钟。

相似文献

1
An improved colloidal silver staining method of protein blots on nitrocellulose membranes.一种改进的用于硝酸纤维素膜上蛋白质印迹的胶体银染色方法。
Folia Biol (Praha). 1987;33(4):253-7.
2
Destaining of nitrocellulose blots after staining with silver or colloidal gold.用银或胶体金染色后硝酸纤维素印迹的脱色。
Anal Biochem. 1993 Oct;214(1):325-8. doi: 10.1006/abio.1993.1496.
3
Quantification of proteins in sample buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using colloidal silver.使用胶体银对十二烷基硫酸钠-聚丙烯酰胺凝胶电泳样品缓冲液中的蛋白质进行定量分析。
Electrophoresis. 1991 Jun;12(6):453-6. doi: 10.1002/elps.1150120617.
4
Sensitive colloidal metal (gold or silver) staining of protein blots on nitrocellulose membranes.
Anal Biochem. 1985 Mar;145(2):315-21. doi: 10.1016/0003-2697(85)90368-9.
5
Silver staining of proteins on electroblotting membranes and intensification of silver staining of proteins separated by polyacrylamide gel electrophoresis.蛋白质在电转印膜上的银染以及聚丙烯酰胺凝胶电泳分离的蛋白质银染的增强。
Anal Biochem. 2002 May 1;304(1):33-41. doi: 10.1006/abio.2001.5604.
6
Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels.用于PhastSystem显影装置快速染色的改良银染程序。I. 十二烷基硫酸钠凝胶的染色
Electrophoresis. 1988 Jan;9(1):28-32. doi: 10.1002/elps.1150090106.
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Detection of immobilized proteins on nitrocellulose membranes using a biotinylation-dependent system.使用生物素化依赖系统检测硝酸纤维素膜上固定的蛋白质。
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Direct Blue 71 staining of proteins bound to blotting membranes.与印迹膜结合的蛋白质的直接蓝71染色。
Electrophoresis. 2000 Mar;21(5):841-5. doi: 10.1002/(SICI)1522-2683(20000301)21:5<841::AID-ELPS841>3.0.CO;2-4.
9
[Detection by the use of silver ions of additional protein zones in gradient polyacrylamide gels stained with Coomassie].[利用银离子检测考马斯亮蓝染色的梯度聚丙烯酰胺凝胶中的额外蛋白区带]
Nauchnye Doki Vyss Shkoly Biol Nauki. 1988(5):101-5.
10
Preparation of colloidal gold for staining proteins electrotransferred onto nitrocellulose membranes.
Anal Biochem. 1988 Jul;172(1):104-7. doi: 10.1016/0003-2697(88)90417-4.

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