Orbán L, Hahn E, Chrambach A
Section on Macromolecular Analysis, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Electrophoresis. 1988 Apr;9(4):167-71. doi: 10.1002/elps.1150090404.
Discontinuous buffer systems operative between pH 5.7 and 7.4, 0 degrees C, were generated, which are characterized by more rapidly displaced moving boundaries than applied previously. These allow one to resolve subcellular particles relatively rapidly and at relatively low agarose gel concentrations. A commercial mixture of DNA restriction fragments pre-stained with ethidium bromide was found to be a suitable tracking dye for these boundaries.
在0摄氏度下，生成了pH值在5.7至7.4之间运行的不连续缓冲系统，其特点是移动边界的位移比以前应用的更快。这些系统能够让人们在相对较低的琼脂糖凝胶浓度下相对快速地分离亚细胞颗粒。发现一种预先用溴化乙锭染色的DNA限制性片段商业混合物是这些边界的合适追踪染料。
