State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210008, China.
Nanoscale Res Lett. 2014 Mar 25;9(1):146. doi: 10.1186/1556-276X-9-146.
A synthetic method to prepare a core-shell-structured Fe3O4@SiO2 as a safe nanovehicle for tumor cell targeting has been developed. Superparamagnetic iron oxide is encapsulated inside nonporous silica as the core to provide magnetic targeting. Carboxymethyl chitosan-folic acid (OCMCS-FA) synthesized through coupling folic acid (FA) with OCMCS is then covalently linked to the silica shell and renders new and improved functions because of the original biocompatible properties of OCMCS and the targeting efficacy of FA. Cellular uptake of the nanovehicle was assayed by confocal laser scanning microscope using rhodamine B (RB) as a fluorescent marker in HeLa cells. The results show that the surface modification of the core-shell silica nanovehicle with OCMCS-FA enhances the internalization of nanovehicle to HeLa cells which over-express the folate receptor. The cell viability assay demonstrated that Fe3O4@SiO2-OCMCS-FA nanovehicle has low toxicity and can be used as an eligible candidate for drug delivery system. These unique advantages make the prepared core-shell nanovehicle promising for cancer-specific targeting and therapy.
一种制备核壳结构 Fe3O4@SiO2 的合成方法，用作肿瘤细胞靶向的安全纳米载体。超顺磁性氧化铁被包裹在无孔硅壳内作为核心，提供磁靶向。通过将叶酸（FA）与 OCMCS 偶联合成的羧甲基壳聚糖-叶酸（OCMCS-FA）然后通过共价键连接到硅壳上，并由于 OCMCS 的原始生物相容性和 FA 的靶向功效而赋予新的和改进的功能。使用罗丹明 B（RB）作为荧光标记物，通过共聚焦激光扫描显微镜测定纳米载体的细胞摄取。结果表明，用 OCMCS-FA 对核壳硅纳米载体进行表面修饰，增强了纳米载体向过表达叶酸受体的 HeLa 细胞的内化。细胞活力测定表明，Fe3O4@SiO2-OCMCS-FA 纳米载体毒性低，可作为药物输送系统的合格候选物。这些独特的优势使得制备的核壳纳米载体有望用于癌症特异性靶向治疗。
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