College of Pharmaceutical and Chemical Engineering, Taizhou University, Jiaojiang 318000, People's Republic of China.
Key Laboratory of Auxiliary Chemistry and Technology for Chemical Industry, Ministry of Education, Shaanxi University of Science and Technology, Xi'an 710021, China.
Nanoscale Res Lett. 2014 Oct 8;9(1):558. doi: 10.1186/1556-276X-9-558. eCollection 2014.
In this paper, we investigated the functional imaging properties of magnetic microspheres composed of magnetic core and CdTe quantum dots in the silica shell functionalized with folic acid (FA). The preparation procedure included the preparation of chitosan-coated Fe3O4 nanoparticles (CS-coated Fe3O4 NPs) prepared by a one-pot solvothermal method, the reaction between carboxylic and amino groups under activation of NHS and EDC in order to obtain the CdTe-CS-coated Fe3O4 NPs, and finally the growth of SiO2 shell vent the photoluminescence (PL) quenching via a Stöber method (Fe3O4-CdTe@SiO2). Moreover, in order to have a specific targeting capacity, the magnetic and fluorescent bifunctional microspheres were synthesized by bonding of SiO2 shell with FA molecules via amide reaction (Fe3O4-CdTe@SiO2-FA). The morphology, size, chemical components, and magnetic property of as-prepared composite nanoparticles were characterized by ultraviolet-visible spectroscopy, fluorescent spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), scanning transmission electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM), respectively. The results show that the magnetic and fluorescent bifunctional microspheres have strong luminescent which will be employed for immuno-labeling and fluorescent imaging of HeLa cells.
在本文中，我们研究了由磁性核和 CdTe 量子点组成的磁性微球在功能化叶酸（FA）的二氧化硅壳中的功能成像特性。制备过程包括通过一锅溶剂热法制备壳聚糖包覆的 Fe3O4 纳米粒子（CS-coated Fe3O4 NPs），通过 NHS 和 EDC 激活羧基和氨基之间的反应以获得 CdTe-CS-coated Fe3O4 NPs，最后通过 Stöber 法生长 SiO2 壳以防止光致发光（PL）猝灭（Fe3O4-CdTe@SiO2）。此外，为了具有特定的靶向能力，通过酰胺反应将磁性和荧光双功能微球通过 SiO2 壳与 FA 分子键合来合成（Fe3O4-CdTe@SiO2-FA）。通过紫外-可见光谱、荧光光谱、傅里叶变换红外光谱（FTIR）、X 射线粉末衍射（XRD）、扫描透射电子显微镜（SEM）、透射电子显微镜（TEM）、热重分析（TGA）和振动样品磁强计（VSM）分别对所得复合纳米粒子的形貌、尺寸、化学成分和磁性进行了表征。结果表明，磁性和荧光双功能微球具有很强的发光性，将用于 HeLa 细胞的免疫标记和荧光成像。
