Motojima H, Kobayashi T, Shimane M, Kamachi S, Fukushima M
Applied Research Laboratories, Chugai Pharmaceutical Co., Ltd., Tokyo, Japan.
J Immunol Methods. 1989 Mar 31;118(2):187-92. doi: 10.1016/0022-1759(89)90005-7.
Human granulocyte colony stimulating factor (G-CSF) was detected at the pM level by a simple sequential sandwich enzyme immunoassay. The polyclonal antiserum obtained by immunizing rabbits with recombinant G-CSF did not recognize other colony stimulating factors. The EIA developed gave satisfactory reproducibility as judged by an intra-assay precision of 4.3-6.2% and an interassay precision of 6.2-9.0%. Results from this assay procedure correlated well with those of a bioassay. The method could be performed within 6 h.
通过一种简单的顺序夹心酶免疫测定法在皮摩尔水平检测到人粒细胞集落刺激因子（G-CSF）。用重组G-CSF免疫兔子获得的多克隆抗血清不识别其他集落刺激因子。所开发的酶免疫测定法具有令人满意的重现性，批内精密度为4.3-6.2%，批间精密度为6.2-9.0%。该测定程序的结果与生物测定法的结果相关性良好。该方法可在6小时内完成。
