Bosnes V, Halvorsen R, Gaudernack G, Thorsby E
Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
J Immunol Methods. 1989 Mar 31;118(2):251-5. doi: 10.1016/0022-1759(89)90013-6.
We have developed a simple method for isolation of functionally active T cell receptor (TCR)-gamma delta positive cells from human peripheral blood, using immunomagnetic separation techniques. After culture with feeder cells and interleukin-2, cells thus isolated showed a CD3+ TCR-alpha beta- phenotype, and stained with antibodies against the TCR-gamma delta complex. The TCR-gamma delta+ cells were functionally active, responding with DNA synthesis when stimulated via their CD3 and CD2 molecules in concert, or when interleukin-2 was added.
我们已经开发出一种简单的方法，利用免疫磁珠分离技术从人外周血中分离功能活性T细胞受体（TCR）γδ阳性细胞。在用饲养细胞和白细胞介素-2培养后，如此分离出的细胞呈现CD3+ TCR-αβ-表型，并用抗TCR-γδ复合体的抗体染色。TCR-γδ+细胞具有功能活性，当通过其CD3和CD2分子协同刺激或添加白细胞介素-2时，会以DNA合成做出反应。
