Abe R, Hodes R J
Immunology Branch, National Cancer Institute, Bethesda, Maryland 20892.
J Immunogenet. 1988 Feb-Jun;15(1-3):11-9. doi: 10.1111/j.1744-313x.1988.tb00403.x.
The relationship among different minor lymphocyte stimulatory locus (Mls) determinants, Mlsa, Mlsb, Mlsc and Mlsd, remains unclear. Because of the high degree of cross-reactivity between Mlsa and Mlsd determinants, the weak stimulatory capacity of Mlsc, and the generally non-stimulatory nature of Mlsb, some investigators have recently suggested that Mls is composed of only a single expressed allele originally defined as the a and d alleles. In order to clarify the nature of Mls determinants, T cell clones positively selected for reactivity to the three stimulatory Mls determinants, Mlsa, Mlsc and Mlsd, were generated and their specificities defined by extensive genetic studies. The response pattern of these Mls-specific clones as well as the results of primary mixed lymphocyte responses indicated that: (i) Mlsa and Mlsc determinants recognized by T cells are distinct, demonstrating that polymorphism does exist within the Mls system; and (ii) Mlsd is not an independent Mls type since Mlsd determinants are composed of Mlsa and Mlsc determinants and anti-Mlsd T cell responses are, in fact, the sum of anti-Mlsa and anti-Mlsc responses.
不同的次要淋巴细胞刺激位点（Mls）决定簇，即Mlsa、Mlsb、Mlsc和Mlsd之间的关系仍不清楚。由于Mlsa和Mlsd决定簇之间存在高度交叉反应性，Mlsc的刺激能力较弱，且Mlsb通常无刺激性质，一些研究者最近提出Mls仅由最初定义为a和d等位基因的单个表达等位基因组成。为了阐明Mls决定簇的性质，我们生成了对三种刺激性Mls决定簇Mlsa、Mlsc和Mlsd具有反应性的阳性选择T细胞克隆，并通过广泛的遗传学研究确定了它们的特异性。这些Mls特异性克隆的反应模式以及初次混合淋巴细胞反应的结果表明：（i）T细胞识别的Mlsa和Mlsc决定簇是不同的，这表明Mls系统内确实存在多态性；（ii）Mlsd不是一种独立的Mls类型，因为Mlsd决定簇由Mlsa和Mlsc决定簇组成，抗Mlsd T细胞反应实际上是抗Mlsa和抗Mlsc反应的总和。
