Ciepiela Olga, Zawadzka-Krajewska Anna, Kotuła Iwona, van Overveld Frans, Kulus Marek, Demkow Urszula
Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Medical University of Warsaw , Poland .
Department of Pediatric Pneumonology and Allergology, Medical University of Warsaw , Poland .
Pediatr Allergy Immunol Pulmonol. 2014 Mar 1;27(1):17-23. doi: 10.1089/ped.2014.0328.
Allergen-specific immunotherapy (SIT) is the unique modifying treatment of atopic diseases. Dysregulation of T-cell apoptosis plays a crucial role in the in the development of asthma. Nevertheless, the effect of sublingual immunotherapy (SLIT) on T-cell apoptosis has not been elucidated. The aim of the study was to evaluate the influence of 1 year of SIT in atopic children on the frequency of Th1 and Th2 cells in peripheral blood, on T-cell apoptosis, and on the response of basophils to allergen challenge. Children suffering from bronchial asthma were treated with SLIT for 12 months (Staloral 300). Basophil activation was evaluated by measurement of CD203c antigen expression. Th1 and Th2 cells frequencies and their associated frequencies of apoptosis by the expression of Bcl-2 were evaluated with flow cytometry. Basophil activation showed no difference in response before and after therapy. The frequency of Th1 cells increased (=0.01, =19), whereas the frequency of Th2 cells remained stable. Additionally, a significant increase of Bcl-2 positive Th1 cells was found (=0.0465, =19). We conclude that the increase in frequency of Th1 cells secondary to SLIT might be associated with increased resistance to apoptotic signals. The basophil activation is not useful in evaluation of patient desensitization.
变应原特异性免疫疗法（SIT）是特应性疾病独特的改善性治疗方法。T细胞凋亡失调在哮喘的发展中起关键作用。然而，舌下免疫疗法（SLIT）对T细胞凋亡的影响尚未阐明。本研究的目的是评估1年SIT对特应性儿童外周血中Th1和Th2细胞频率、T细胞凋亡以及嗜碱性粒细胞对变应原激发反应的影响。患有支气管哮喘的儿童接受SLIT治疗12个月（Staloral 300）。通过测量CD203c抗原表达评估嗜碱性粒细胞活化。用流式细胞术评估Th1和Th2细胞频率及其通过Bcl-2表达的相关凋亡频率。治疗前后嗜碱性粒细胞活化反应无差异。Th1细胞频率增加（P = 0.01，n = 19），而Th2细胞频率保持稳定。此外，发现Bcl-2阳性Th1细胞显著增加（P = 0.0465，n = 19）。我们得出结论，SLIT继发的Th1细胞频率增加可能与对凋亡信号的抗性增加有关。嗜碱性粒细胞活化在评估患者脱敏方面无用。
