Lenzi Tathiane Larissa, Tedesco Tamara Kerber, Calvo Ana Flávia, Ricci Hérica Adad, Hebling Josimeri, Raggio Daniela Prócida
J Adhes Dent. 2014 Aug;16(4):333-8. doi: 10.3290/j.jad.a31799.
To evaluate the effect of chemical and microbiological methods of caries induction on microtensile bond strength (μTBS) of current adhesive systems to primary dentin.
Flat dentin surfaces from 36 primary molars were assigned to 3 groups according to the method of inducing caries-affected dentin: (1) control (sound dentin); (2) pH cycling; and (3) microbiological. In both methods, teeth were submitted to caries induction for 14 days, and the sound dentin was stored in distilled water for the same period. Specimens were then randomly reassigned according to adhesive system: a two-step etch-and-rinse adhesive (Adper Single Bond 2) or a two-step self-etching system (Clearfil SE Bond). Composite buildups were constructed and the teeth were sectioned to obtain bonded sticks (0.8 mm2) to be tested for microtensile bond strength. The μTBS means were analyzed by two-way ANOVA and Tukey's tests (α = 0.05). Failure mode was evaluated using a stereomicroscope (400X).
Both methods of caries induction resulted in lower μTBS values (with no significant difference between them) than those obtained for sound dentin. Adhesive systems showed similar bond strength values. The percentage of premature failure was higher in the microbiological group, regardless of adhesive system.
Microbiological and pH-cycling methods are both suitable for simulating caries-affected dentin for bonding evaluations in primary teeth.
评估化学和微生物诱导龋齿方法对当前粘结系统与乳牙牙本质微拉伸粘结强度（μTBS）的影响。
根据诱导龋坏牙本质的方法，将36颗乳牙的平坦牙本质表面分为3组：（1）对照组（健康牙本质）；（2）pH循环组；（3）微生物组。在两种方法中，牙齿均进行14天的龋齿诱导，健康牙本质在蒸馏水中保存相同时间。然后根据粘结系统将标本随机重新分组：一种两步酸蚀冲洗粘结剂（Adper Single Bond 2）或一种两步自酸蚀系统（Clearfil SE Bond）。构建复合树脂充填体，将牙齿切片以获得粘结棒（0.8平方毫米），用于测试微拉伸粘结强度。通过双向方差分析和Tukey检验（α = 0.05）分析μTBS均值。使用体视显微镜（400倍）评估失败模式。
两种龋齿诱导方法导致的μTBS值均低于健康牙本质（两者之间无显著差异）。粘结系统显示出相似的粘结强度值。无论粘结系统如何，微生物组的早期失败百分比均较高。
微生物法和pH循环法均适用于模拟乳牙龋坏牙本质以进行粘结评估。
