J Adhes Dent. 2013 Oct;15(5):439-45. doi: 10.3290/j.jad.a28880.
To evaluate the bonding of simplified adhesive systems to sound and caries-affected dentin of primary teeth with microtensile (µTBS) and nanoleakage (NL) tests.
Occlusal cavities were prepared in 36 sound second primary molars. Half of the specimens were submitted to pH cycling to simulate caries-affected dentin. Teeth were randomly restored with one of three materials: the etch-and-rinse adhesive system Adper Single Bond 2 (SB), the two-step self-etching adhesive system Adper SE Plus (SE), and the one-step self-etching adhesive system Adper Easy One (EASY). After storage for 24 h, specimens with cross-sectional areas of 0.8 mm2 were prepared for microtensile testing (1 mm/min). One stick from each tooth was immersed in silver nitrate solution (24 h) and allowed to develop for 8 h in order to score the nano leakage with SEM. The fracture pattern was evaluated using a stereomicroscope (400X). The µTBS means were analyzed by two-way ANOVA and Tukey's post-hoc test. For NL, the Kruskal- Wallis and Mann-Whitney tests were used (α < 0.05).
SB (35.5 ± 3.5) showed the highest µTBS value to sound dentin, followed by EASY (26.3 ± 1.9) and SE (18.2 ± 6.5) (p < 0.05). No difference among materials was observed for caries-affected dentin (SB: 17.8 ± 4.2; SE: 13.9 ± 3.2; EASY: 14.4 ± 4.2, p > 0.05). For all groups, adhesive/mixed fracture prevailed. Caries affected dentin promoted silver nitrate uptake into the adhesive interface; however, with SE, the nano leakage was more pronounced than in the other adhesive systems, even in sound dentin.
Caries-affected dentin negatively influences the bond strength and nano leakage of the two-step etch-and-rinse and one-step self-etching adhesive systems tested in primary teeth.
通过微拉伸(µTBS)和纳米渗漏(NL)试验评估简化型黏结系统与乳牙健康和龋损牙本质的黏结。
在 36 颗健康的第二乳磨牙上制备咬合面窝洞。将一半样本进行 pH 循环以模拟龋损牙本质。牙齿随机用三种材料之一进行修复:酸蚀-冲洗黏结剂 Adper Single Bond 2(SB)、两步自酸蚀黏结剂 Adper SE Plus(SE)和一步自酸蚀黏结剂 Adper Easy One(EASY)。在储存 24 h 后,制备 0.8 mm2 横截面积的试件进行微拉伸测试(1 mm/min)。从每个牙齿上取一个棒,浸入硝酸银溶液中(24 h),并在 SEM 下允许发展 8 h 以记录纳米渗漏。使用立体显微镜(400X)评估断裂模式。通过双因素方差分析和 Tukey 事后检验分析 µTBS 平均值。对于 NL,使用 Kruskal-Wallis 和 Mann-Whitney 检验(α<0.05)。
SB(35.5 ± 3.5)对健康牙本质的 µTBS 值最高,其次是 EASY(26.3 ± 1.9)和 SE(18.2 ± 6.5)(p<0.05)。在龋损牙本质中,三种材料之间没有差异(SB:17.8 ± 4.2;SE:13.9 ± 3.2;EASY:14.4 ± 4.2,p>0.05)。对于所有组,均以黏结/混合断裂为主。龋损牙本质促进硝酸银进入黏结界面,但与 SE 相比,即使在健康牙本质中,其纳米渗漏也更为明显。
龋损牙本质对乳牙中测试的两步酸蚀-冲洗和一步自酸蚀黏结系统的黏结强度和纳米渗漏有负面影响。