鉴定与B220及其他T200限制性抗体发生反应所需的小鼠CD45（T200）可变剪接外显子。
Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies.
作者信息
Johnson P, Greenbaum L, Bottomly K, Trowbridge I S
机构信息
Department of Cancer Biology, Salk Institute, San Diego, California 92138.
出版信息
J Exp Med. 1989 Mar 1;169(3):1179-84. doi: 10.1084/jem.169.3.1179.
Abstract
Cell lines expressing specific variants of murine CD45 (T200) were established by infection with retroviral constructs of four cDNAs encoding different forms of CD45. These lines were then used to determine which sequences encoded by alternatively spliced exons of CD45 were required to generate antigenic determinants recognized by anti-CD45 mAbs. The binding of two B220 antibodies (14.8 and RA32C2) to CD45 was dependent on the expression of the first alternatively spliced exon (exon A). A third B220 antibody, RA3-6B2, did not bind to any of the forms of CD45 expressed on fibroblasts. A newly defined anti-CD45R antibody, C363.16A, reacts with an antigenic site dependent upon the expression of the second alternatively spliced exon, exon B.
摘要
通过用编码不同形式CD45的四个cDNA的逆转录病毒构建体感染，建立了表达小鼠CD45（T200）特定变体的细胞系。然后利用这些细胞系来确定CD45可变剪接外显子编码的哪些序列是产生抗CD45单克隆抗体识别的抗原决定簇所必需的。两种B220抗体（14.8和RA32C2）与CD45的结合依赖于第一个可变剪接外显子（外显子A）的表达。第三种B220抗体RA3-6B2不与成纤维细胞上表达的任何形式的CD45结合。一种新定义的抗CD45R抗体C363.16A与一个依赖于第二个可变剪接外显子外显子B表达的抗原位点发生反应。
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