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蜱染色体的差异染色:血红扇头蜱(拉特雷尔)的C带和银染技术及核型分析

Differential staining of tick chromosomes: techniques for C-banding and silver-staining and karyology of Rhipicephalus sanguineus (Latreille).

作者信息

Gunn S J, Hilburn L R

机构信息

U.S. Department of Agriculture, Agricultural Research Service, U.S. Livestock Insects Laboratory, Kerrville, Texas 78028-0232.

出版信息

J Parasitol. 1989 Apr;75(2):239-45.

PMID:2466974
Abstract

A new karyotypic procedure is described for tick chromosomes along with modifications of accepted techniques for C-banding and silver-staining. The staining procedures produce patterns with staining limited to the centromeric regions and the nucleolar organizer regions (NORs). The possible reasons for the concordant patterns are discussed based on the staining characteristics of the 2 techniques. The karyotype of Rhipicephalus sanguineus possesses 20 acrocentric autosomes with a single pair of NORs located proximal to the centromere on the largest autosomal pair. The sex-determining system is XX:XO with the X chromosome being the largest chromosome in the complement and subtelocentric.

摘要

本文描述了一种用于蜱虫染色体的新核型分析方法,以及对公认的C带染色和银染技术的改进。这些染色程序产生的模式中,染色仅限于着丝粒区域和核仁组织区(NORs)。基于这两种技术的染色特性,讨论了出现一致模式的可能原因。血红扇头蜱的核型有20条近端着丝粒常染色体,在最大的常染色体对中,有一对NORs位于着丝粒近端。其性别决定系统为XX:XO,X染色体是染色体组中最大的染色体,为亚端着丝粒染色体。

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