Anderson G R, Stoler D L, Scarcello L A
Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, Buffalo, NY 14263.
J Mol Biol. 1989 Feb 20;205(4):765-9. doi: 10.1016/0022-2836(89)90320-3.
VL30 elements are a multigene family within the class of retroviruses and retrotransposons. We have characterized the response of normal rat fibroblasts to anoxia, in which endogenous VL30 element expression is strongly induced. Optimal induction up to 500-fold occurs under complete anoxia, although a lesser response is seen under atmospheres up to 2% oxygen. Phorbol esters and diacylglycerol, which induce mouse VL30 RNA approximately eightfold, show no effect on the rat VL30 system. The hypoxic conditions optimal for rat VL30 induction represent a mild cellular stress, with no reduction in cell viability during the induction period. Although the precise physiological role of this fibroblast response to temporary anoxia is unknown, it may occur during wound healing. The induction of VL30 by anoxia provides a unique model system wherein a member of the mammalian retrovirus/retrotransposon family is highly responsive to a common physiological signal.
VL30元件是逆转录病毒和逆转座子类中的一个多基因家族。我们已经描述了正常大鼠成纤维细胞对缺氧的反应，其中内源性VL30元件的表达被强烈诱导。在完全缺氧的情况下，诱导作用最佳，可达500倍，不过在含氧量高达2%的环境中，反应较小。能诱导小鼠VL30 RNA增加约8倍的佛波酯和二酰基甘油，对大鼠VL30系统没有影响。对大鼠VL30诱导作用最佳的缺氧条件代表一种轻度的细胞应激，在诱导期细胞活力没有降低。尽管这种成纤维细胞对暂时缺氧反应的确切生理作用尚不清楚，但它可能发生在伤口愈合过程中。缺氧诱导VL30提供了一个独特的模型系统，其中哺乳动物逆转录病毒/逆转座子家族的一个成员对一种常见的生理信号高度敏感。
