Osafi Javid, Hejazi Ali, Stutz Derek D, Keiserman Mark A, Bergman Christine J, Kingsley Karl
1School of Dental Medicine, University of Nevada, Las Vegas-Las Vegas, Nevada, USA.
J Diet Suppl. 2014 Jun;11(2):145-54. doi: 10.3109/19390211.2013.859209.
The inverse association between vitamin D and cancer risk is well-established, but the relationship with oral cancer is less well-understood. To further the understanding of these relationships, this study sought to evaluate any growth-inhibiting effects of vitamin D on well-characterized oral cancers.
This study utilized 1,25-dihydroxy Vitamin D3 to evaluate any changes in growth using CAL27, SCC15, and SCC25 oral cancer cell lines at physiological and supraphysiological concentrations.
These assays revealed that the growth of all three cancer cell lines was significantly reduced by vitamin D administration, with maximal inhibition in SCC15 of -6.8% at 50 nmol, -19.7% in CAL27, and -43.6% in SCC25 at 100 nmol (p < .05). In addition, the observed decreases in growth were associated with significant decreases in viability (ranging from -18% in SCC15, -23% in CAL27, and -47% in SCC25 cells), as well as activation of two key apoptotic pathways (caspase and bcl:bax).
The results of this study demonstrate the growth-inhibitory effects of vitamin D administration in specific oral cancer cell lines, which will enhance the understanding of oral oncologists and oral health researchers in developing standards for generalizing the health-protective effects of diet and dietary supplements as treatment options for patients with oral cancer.
维生素D与癌症风险之间的负相关关系已得到充分证实，但与口腔癌的关系尚不太清楚。为了进一步了解这些关系，本研究旨在评估维生素D对特征明确的口腔癌的任何生长抑制作用。
本研究使用1,25 - 二羟基维生素D3，在生理和超生理浓度下，利用CAL27、SCC15和SCC25口腔癌细胞系评估生长的任何变化。
这些试验表明，给予维生素D后，所有三种癌细胞系的生长均显著降低，在50 nmol时，SCC15的最大抑制率为 - 6.8%，CAL27为 - 19.7%，在100 nmol时，SCC25为 - 43.6%（p < 0.05）。此外，观察到的生长下降与活力的显著下降相关（SCC15中为 - 18%，CAL27中为 - 23%，SCC25细胞中为 - 47%），以及两条关键凋亡途径（半胱天冬酶和bcl:bax）的激活。
本研究结果证明了给予维生素D对特定口腔癌细胞系具有生长抑制作用，这将增进口腔肿瘤学家和口腔健康研究人员对制定标准的理解，以便将饮食和膳食补充剂的健康保护作用推广为口腔癌患者的治疗选择。
