Shi Pengwei, Gao Yanbin, Lu Zhiyang, Yang Lei
Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Mar;34(3):426-9.
To investigate the effect of antibacterial peptide LL-37 on the integrity of Acinetobacter baumannii biofilm.
A model of Acinetobacter baumannii biofilm in vitro was constructed by plates and crystal violet staining method, and the minimal inhibitory concentration of LL-37 was determined by broth dilution. The biofilm morphology was observed by scanning electron microscopy and biofilm formation was analyzed by the crystal violet staining of the adherent biofilm in the presence of antibacterial peptide LL-37.
In the Acinetobacter baumannii biofilm model, the minimal inhibitory concentration of LL-37 was 64 µg/ml; LL-37 caused structural damage of the biofilm at a low concentration of 2.5 µg/ml. The biofilm was decreased gradually as the concentration of LL-37 increased.
LL-37 even at a concentration far below its minimal inhibitory concentration can cause structural damage of Acinetobacter baumannii biofilm in vitro.
探讨抗菌肽LL-37对鲍曼不动杆菌生物膜完整性的影响。
采用平板法和结晶紫染色法构建鲍曼不动杆菌体外生物膜模型，采用肉汤稀释法测定LL-37的最低抑菌浓度。通过扫描电子显微镜观察生物膜形态，并采用结晶紫染色法分析在抗菌肽LL-37存在下黏附生物膜的生物膜形成情况。
在鲍曼不动杆菌生物膜模型中，LL-37的最低抑菌浓度为64μg/ml；LL-37在2.5μg/ml的低浓度下即可引起生物膜结构损伤。随着LL-37浓度的增加，生物膜逐渐减少。
即使在远低于其最低抑菌浓度的情况下，LL-37也可在体外引起鲍曼不动杆菌生物膜的结构损伤。
