Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.
Probiotics Antimicrob Proteins. 2019 Mar;11(1):317-324. doi: 10.1007/s12602-018-9444-5.
Acinetobacter baumannii is one of the most challenging pathogens, on account of its predisposition to develop resistance leading to severe, difficult-to-treat infections. As these bacteria are more usually isolated from nosocomial infections, the new therapeutic options are demanded. Antimicrobial peptides (AMPs) are compounds likely to find application in the treatment of A. baumannii. These compounds exhibit a wide spectrum of antimicrobial activity and were found to be effective against biofilm. In this study, eight AMPs, namely aurein 1.2, CAMEL, citropin 1.1., LL-37, omiganan, r-omiganan, pexiganan, and temporin A, were tested for their antimicrobial activity. A reference strain of A. baumannii ATCC 19606 was used. Antimicrobial assays included determination of the minimum inhibitory concentration and the minimum biofilm eradication concentration. Considering the fact that the majority of A. baumannii infections are associated with mechanical ventilation and the use of indwelling devices, the activity against biofilm was assessed on both a polystyrene surface and tracheal tube fragments. In addition, cytotoxicity (HaCaT) was determined and in vitro selectivity index was calculated.
鲍曼不动杆菌是最具挑战性的病原体之一，由于其易产生耐药性，导致严重的、难以治疗的感染。由于这些细菌通常从医院感染中分离出来，因此需要新的治疗选择。抗菌肽（AMPs）是一种可能在治疗鲍曼不动杆菌方面有应用前景的化合物。这些化合物表现出广谱的抗菌活性，并被发现对生物膜有效。在这项研究中，测试了 8 种 AMPs，即 aurein 1.2、CAMEL、citropin 1.1、LL-37、omiganan、r-omiganan、pexiganan 和 temporin A，以评估它们的抗菌活性。使用了鲍曼不动杆菌 ATCC 19606 的参考菌株。抗菌测定包括最小抑菌浓度和最小生物膜清除浓度的测定。考虑到大多数鲍曼不动杆菌感染与机械通气和留置装置的使用有关，因此在聚苯乙烯表面和气管管段上评估了对生物膜的活性。此外，还测定了细胞毒性（HaCaT）并计算了体外选择性指数。
