Hossain Md Motaher, Sultana Farjana, Miyazawa Mitsuo, Hyakumachi Mitsuro
United Graduate School of Agricultural Sciences, Gifu University.
J Oleo Sci. 2014;63(4):391-400. doi: 10.5650/jos.ess13143.
Plant growth-promoting fungi (PGPF) have the potential to confer several benefits to plants in terms of growth and protection against pests and pathogens. In the present study, we tested whether a PGPF isolate, Penicillium spp. GP15-1 (derived from zoysiagrass rhizospheres), stimulates growth and disease resistance in the cucumber plant. The use of the barley grain inoculum GP15-1 significantly enhanced root and shoot growth and biomass of cucumber plants. A root colonization study revealed that GP15-1 was a very rapid and efficient root colonizer and was isolated in significantly higher frequencies from the upper root parts than from the middle and lower root parts during the first 14 d of seedling growth. Inoculating the cucumber seedlings with GP15-1 significantly reduced the damping-off disease caused by Rhizoctonia solani, and the disease suppression effects of GP15-1 were considerably influenced by the inoculum potential of both GP15-1 and the pathogen. Treatment with the barley grain inoculum or a cell-free filtrate of GP15-1 increased systemic resistance against leaf infection by the anthracnose pathogen Colletotrichum orbiculare, resulting in a significant decrease in lesion number and size. Molecular and phylogenetic analyses of internal transcribed spacer sequences of the genomic DNA of GP15-1 revealed that the fungal isolate is a strain of either Penicillium neoechinulatum or Penicillium viridicatum.
植物促生真菌（PGPF）在促进植物生长以及保护植物免受病虫害侵害方面具有多种潜在益处。在本研究中，我们测试了一种PGPF分离株，即青霉属GP15-1（源自结缕草根际），是否能促进黄瓜植株的生长并增强其抗病性。使用大麦粒接种物GP15-1显著促进了黄瓜植株的根和地上部生长以及生物量。一项根系定殖研究表明，GP15-1是一种定殖速度非常快且效率高的根系定殖真菌，在幼苗生长的前14天内，从根上部被分离出的频率显著高于根中部和下部。用GP15-1接种黄瓜幼苗显著降低了由立枯丝核菌引起的猝倒病，GP15-1的病害抑制效果受其自身和病原菌接种量的显著影响。用大麦粒接种物或GP15-1的无细胞滤液处理可增强对炭疽病菌（Colletotrichum orbiculare）叶部感染的系统抗性，导致病斑数量和大小显著减少。对GP15-1基因组DNA的内部转录间隔区序列进行分子和系统发育分析表明，该真菌分离株是新刺青霉（Penicillium neoechinulatum）或绿青霉（Penicillium viridicatum）的一个菌株。
