Lin Chiahsin, Kuo Fu-Wen, Chavanich Suchana, Viyakarn Voranop
National Museum of Marine Biology & Aquarium, Checheng, Pingtung, Taiwan; Institute of Marine Biotechnology, National Dong Hwa University, Checheng, Pingtung, Taiwan.
National Museum of Marine Biology & Aquarium, Checheng, Pingtung, Taiwan.
PLoS One. 2014 Mar 26;9(3):e92812. doi: 10.1371/journal.pone.0092812. eCollection 2014.
The lipid phase transition (LPT) from the fluid liquid crystalline phase to the more rigid gel structure phase that occurs upon exposure to low temperatures can affect physical structure and function of cellular membranes. This study set out to investigate the membrane phase behavior of oocytes of three gorgonian corals; Junceela fragilis, J. juncea and Ellisella robusta,at different developmental stages after exposure to reduced temperatures. Oocytes were chilled to 5°C for 48, 96 or 144 h, and the LPT temperature (LPTT) was determined with Fourier Transform Infrared (FTIR) spectroscopy. The J. fragilis oocytes had a higher LPTT (∼23.0-23.7°C) than those of J. juncea and E. robusta oocytes (approximately 18.3-20.3°C). Upon chilling for 96 h at 5°C, the LPTTs of J. juncea and E. robusta oocytes in the early (18.0±1.0 and 18.3±0.6°C, respectively) and late (17.3±0.6 and 17.7±1.2°C, respectively) stages were significantly lower than those of J. fragilis oocytes (20.3±2.1 and 19.3±1.5°C for the early and late stages, respectively). The LPTTs of early stage gorgonian oocytes was significantly lower than those of late stage oocytes. These results suggest that the LPT of three gorgonian oocytes at different developmental stages may have been influenced by the phospholipid composition of their plasma membranes, which could have implications for their low temperature resistance.
暴露于低温时发生的从流体液晶相到更刚性凝胶结构相的脂质相变（LPT）会影响细胞膜的物理结构和功能。本研究旨在调查三种柳珊瑚（脆弱叶柳珊瑚、琼氏柳珊瑚和粗壮艾氏柳珊瑚）卵母细胞在暴露于低温后不同发育阶段的膜相行为。将卵母细胞冷却至5°C，持续48、96或144小时，并用傅里叶变换红外（FTIR）光谱法测定脂质相变温度（LPTT）。脆弱叶柳珊瑚的卵母细胞比琼氏柳珊瑚和粗壮艾氏柳珊瑚的卵母细胞具有更高的LPTT（约23.0 - 23.7°C）（约18.3 - 20.3°C）。在5°C下冷却96小时后，琼氏柳珊瑚和粗壮艾氏柳珊瑚早期（分别为18.0±1.0和18.3±0.6°C）和晚期（分别为17.3±0.6和17.7±1.2°C）卵母细胞的LPTT显著低于脆弱叶柳珊瑚卵母细胞（早期和晚期分别为20.3±2.1和19.3±1.5°C）。早期柳珊瑚卵母细胞的LPTT显著低于晚期卵母细胞。这些结果表明，三种柳珊瑚不同发育阶段卵母细胞的LPT可能受到其质膜磷脂组成的影响，这可能对它们的低温抗性有影响。
