National Museum of Marine Biology and Aquarium, Checheng, Pingtung, Taiwan.
Theriogenology. 2012 Apr 1;77(6):1257-61. doi: 10.1016/j.theriogenology.2011.09.021. Epub 2011 Dec 6.
Understanding chilling sensitivity and chilling injury of coral oocytes, in the presence and absence of a cryoprotectant, is important in developing cryopreservation protocols, as well as for short-term storage and transport (e.g., for species conservation). The objective of this study was to investigate the chilling sensitivity of hard coral (Echinopora spp.) oocytes and the effectiveness of methanol (as a cryoprotectant) in protecting these oocytes during short-term, low temperature preservation. Oocytes were exposed to 0.5, 1, or 2 m methanol at 5, 0, or -5 °C for 1, 2, 4, 6, 8, 16, or 32 h, and their quality determined based on adenosine triphosphate (ATP) content. Methanol at 0.5 m was the most effective means to reduce chilling-induced reduction in ATP concentrations. Coral oocytes can be stored at room temperature for 4 h in filtered nature seawater with no detrimental effect on oocyte quality; however, in the present study, oocyte survival was extended for 8 h by addition of methanol in low concentrations (0.5 or 1 m) at low temperatures (5 and 0 °C). These findings should enhance conservation efforts and facilitate low-temperature transport of endangered and threatened coral species.
了解珊瑚卵母细胞在有或没有冷冻保护剂的情况下的冷藏敏感性和冷藏损伤对于开发冷冻保存方案以及短期储存和运输（例如，用于物种保护）非常重要。本研究的目的是研究硬珊瑚（Echinopora spp.）卵母细胞的冷藏敏感性，以及甲醇（作为冷冻保护剂）在保护这些卵母细胞在短期低温保存期间的有效性。将卵母细胞暴露于 5、0 或-5°C 的 0.5、1 或 2 m 甲醇中 1、2、4、6、8、16 或 32 h，并根据三磷酸腺苷 (ATP) 含量确定其质量。0.5 m 的甲醇是降低冷藏引起的 ATP 浓度降低的最有效方法。珊瑚卵母细胞可以在过滤的自然海水中在室温下储存 4 小时，而不会对卵母细胞质量产生不利影响；然而，在本研究中，通过在低温（5 和 0°C）下添加低浓度（0.5 或 1 m）甲醇，将卵母细胞的存活时间延长了 8 小时。这些发现应该会增强保护工作，并促进濒危和受威胁的珊瑚物种的低温运输。
