Bellstedt Peter, Ihle Yvonne, Wiedemann Christoph, Kirschstein Anika, Herbst Christian, Görlach Matthias, Ramachandran Ramadurai
Leibniz Institute for Age Research - Fritz Lipmann Institute, Department Biomolecular NMR Spectroscopy, Beutenbergstraße 11, 07745 Jena, Germany.
1] Leibniz Institute for Age Research - Fritz Lipmann Institute, Department Biomolecular NMR Spectroscopy, Beutenbergstraße 11, 07745 Jena, Germany [2].
Sci Rep. 2014 Mar 27;4:4490. doi: 10.1038/srep04490.
RF pulse schemes for the simultaneous acquisition of heteronuclear multi-dimensional chemical shift correlation spectra, such as {HA(CA)NH & HA(CACO)NH}, {HA(CA)NH & H(N)CAHA} and {H(N)CAHA & H(CC)NH}, that are commonly employed in the study of moderately-sized protein molecules, have been implemented using dual sequential (1)H acquisitions in the direct dimension. Such an approach is not only beneficial in terms of the reduction of experimental time as compared to data collection via two separate experiments but also facilitates the unambiguous sequential linking of the backbone amino acid residues. The potential of sequential (1)H data acquisition procedure in the study of RNA is also demonstrated here.
用于同时采集异核多维化学位移相关谱的射频脉冲序列，如常用于中等大小蛋白质分子研究的{HA(CA)NH & HA(CACO)NH}、{HA(CA)NH & H(N)CAHA}和{H(N)CAHA & H(CC)NH}，已通过在直接维度上进行双序列¹H采集得以实现。与通过两个单独实验进行数据收集相比，这种方法不仅在减少实验时间方面有益，而且有助于明确主链氨基酸残基的序列连接。本文还展示了序列¹H数据采集程序在RNA研究中的潜力。
