Wiedemann Christoph, Ohlenschläger Oliver, Medagli Barbara, Onesti Silvia, Görlach Matthias
Biomolecular NMR Spectroscopy, Leibniz Institute for Age Research-Fritz Lipman Institute, Beutenbergstr. 11, 07745, Jena, Germany,
Biomol NMR Assign. 2014 Oct;8(2):357-60. doi: 10.1007/s12104-013-9516-0. Epub 2013 Aug 10.
High-fidelity replication guarantees the stable inheritance of genetic information stored in the DNA of living organisms. The minichromosome maintenance (MCM) complex functions as replicative DNA-unwinding helicase and has been identified as one key player in the replication process of archea and eukarya. Despite the availability of considerable structural information on archeal MCMs, such information was missing for their C-terminal domain. In order to obtain more detailed structural information, we assigned the NMR chemical shifts for backbone and side chain nuclei for the MCM C-terminal winged helix domains of the archeal species Methanothermobacter thermautrophicus and Sulfolobus solfataricus.
高保真复制确保了存储在生物体DNA中的遗传信息的稳定遗传。微小染色体维持(MCM)复合物作为复制性DNA解旋螺旋酶发挥作用,并已被确定为古细菌和真核生物复制过程中的一个关键参与者。尽管已有大量关于古细菌MCM的结构信息,但关于其C端结构域的此类信息却缺失。为了获得更详细的结构信息,我们对嗜热栖热甲烷杆菌和嗜热栖热硫化叶菌这两种古细菌物种的MCM C端带翼螺旋结构域的主链和侧链原子核的核磁共振化学位移进行了归属。