Cha Young-Lok, Yang Jungwoo, Ahn Jong-Woong, Moon Youn-Ho, Yoon Young-Mi, Yu Gyeong-Dan, An Gi Hong, Choi In-Hu
Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, 199 Muan-ro, Cheonggye, Muan, 534-833, South Korea.
Bioprocess Biosyst Eng. 2014 Sep;37(9):1907-15. doi: 10.1007/s00449-014-1165-x. Epub 2014 Mar 27.
A CO2-added ammonia explosion pretreatment was performed for bioethanol production from rice straw. The pretreatment conditions, such as ammonia concentration, CO2 loading level, residence time, and temperature were optimized using response surface methodology. The response for optimization was defined as the glucose conversion rate. The optimized pretreatment conditions resulting in maximal glucose yield (93.6 %) were determined as 14.3 % of ammonia concentration, 2.2 MPa of CO2 loading level, 165.1 °C of temperature, and 69.8 min of residence time. Scanning electron microscopy analysis showed that pretreatment of rice straw strongly increased the surface area and pore size, thus increasing enzymatic accessibility for enzymatic saccharification. Finally, an ethanol yield of 97 % was achieved via simultaneous saccharification and fermentation. Thus, the present study suggests that CO2-added ammonia pretreatment is an appropriate process for bioethanol production from rice straw.
采用添加二氧化碳的氨爆预处理法从稻草中生产生物乙醇。利用响应面法对预处理条件，如氨浓度、二氧化碳添加量、停留时间和温度进行了优化。将优化的响应定义为葡萄糖转化率。得到最大葡萄糖产率（93.6%）的优化预处理条件为氨浓度14.3%、二氧化碳添加量2.2MPa、温度165.1℃和停留时间69.8分钟。扫描电子显微镜分析表明，稻草的预处理显著增加了表面积和孔径，从而提高了酶促糖化的酶可及性。最后，通过同步糖化发酵实现了97%的乙醇产率。因此，本研究表明添加二氧化碳的氨预处理是从稻草生产生物乙醇的合适工艺。
