轻度堿预处理可实现稻草转化为生物乙醇的高水解和发酵效率。
Mild alkaline pretreatment can achieve high hydrolytic and fermentation efficiencies for rice straw conversion to bioethanol.
机构信息
Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
Biofuels and Biorefineries Section, Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram, India.
出版信息
Prep Biochem Biotechnol. 2020;50(8):814-819. doi: 10.1080/10826068.2020.1744007. Epub 2020 Mar 24.
Mild alkaline pretreatment was evaluated as a strategy for effective lignin removal and hydrolysis of rice straw. The pretreatment efficiency of different NaOH concentrations (0.5, 1.0, 1.5 or 2.0% w/w) was assessed. Rice straw (RS) pretreated with 1.5% NaOH achieved better sugar yield compared to other concentrations used. A cellulose conversion efficiency of 91% (45.84 mg/ml glucose release) was attained from 1.5% NaOH pretreated rice straw (PRS), whereas 1% NaOH pretreated rice straw yielded 35.10 mg/ml of glucose corresponding to a cellulose conversion efficiency of 73.81%. The ethanol production from 1% and 1.5% NaOH pretreated RS hydrolysates was similar at ∼3.3% (w/v), corresponding to a fermentation efficiency of 86%. The non-detoxified hydrolysate was fermented using the novel yeast strain RPP-03O without any additional supplementation of nutrients.
温和的碱性预处理被评估为一种有效去除木质素和水解稻草的策略。评估了不同 NaOH 浓度（0.5%、1.0%、1.5%或 2.0%w/w）的预处理效率。与使用的其他浓度相比，用 1.5%NaOH 预处理的稻草（RS）的糖产率更高。从 1.5%NaOH 预处理的稻草（PRS）中获得了 91%的纤维素转化率效率（45.84mg/ml 葡萄糖释放），而 1%NaOH 预处理的稻草产生了 35.10mg/ml 的葡萄糖，对应的纤维素转化率效率为 73.81%。用 1%和 1.5%NaOH 预处理的 RS 水解物的乙醇产量相似，约为 3.3%（w/v），对应的发酵效率为 86%。未经解毒的水解物使用新型酵母菌株 RPP-03O 进行发酵，无需额外补充营养物质。
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