Centre for Biofuels, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum, 695 019, India.
World J Microbiol Biotechnol. 2012 Feb;28(2):473-83. doi: 10.1007/s11274-011-0838-8. Epub 2011 Jul 10.
The present study investigates the operational conditions for organosolvent pretreatment and hydrolysis of rice straw. Among the different organic acids and organic solvents tested, acetone was found to be most effective based on the fermentable sugar yield. Optimization of process parameters for acetone pretreatment were carried out. The structural changes before and after pretreatment were investigated by scanning electron microscopy, X-ray diffraction and Fourier transform infrared (FTIR) analysis. The X-ray diffraction profile showed that the degree of crystallinity was higher for acetone pretreated biomass than that of the native. FTIR spectrum also exhibited significant difference between the native and pretreated samples. Under optimum pretreatment conditions 0.458 g of reducing sugar was produced per gram of pretreated biomass with a fermentation efficiency of 39%. Optimization of process parameters for hydrolysis such as biomass loading, enzyme loading, surfactant concentration and incubation time was done using Box-Benhken design. The results indicate that acetone pretreated rice straw can be used as a good feed stock for bioethanol production.
本研究探讨了有机溶剂预处理和水稻秸秆水解的操作条件。在测试的不同有机酸和有机溶剂中，基于可发酵糖产量，发现丙酮最有效。对丙酮预处理的工艺参数进行了优化。通过扫描电子显微镜、X 射线衍射和傅里叶变换红外（FTIR）分析研究了预处理前后的结构变化。X 射线衍射图谱表明，预处理生物质的结晶度高于天然生物质。FTIR 光谱也显示了天然样品和预处理样品之间的显著差异。在最佳预处理条件下，每克预处理生物质可产生 0.458 克还原糖，发酵效率为 39%。通过 Box-Benhken 设计对水解的工艺参数（生物质负荷、酶负荷、表面活性剂浓度和孵育时间）进行了优化。结果表明，丙酮预处理的水稻秸秆可用作生物乙醇生产的良好原料。
