Barracco M A, De Oliveira R, Schlemper Júnior B
Universidade Federal de Santa Catarina, Departamento de Biologia, Florianópolis, Brasil.
Mem Inst Oswaldo Cruz. 1987 Jul-Sep;82(3):431-8. doi: 10.1590/s0074-02761987000300017.
Five hemocyte types were identified in the hemolymph of Panstrongylus megistus by phase contrast and common light microscopy using some histochemical methods. These are: Prohemocytes, small cells presenting a great nucleus/cytoplasm ratio; Plasmatocytes, the most numerous hemocytes, are polymorphic cells mainly characterized by a large amount of lysosomes; Granulocytes, hemocytes very similar to plasmatocytes which contain cytoplasmic granules and are especially rich in polysaccharides; Oenocytoids, cells presenting a small nucleus and a thick cytoplasm; they show many small round vacuoles when observed in Giemsa smears and many cytoplasmic granules under phase microscopy; Adipohemocytes, very large hemocytes, presenting many fat droplet inclusions which could correspond to free fat bodies which entered the hemolymph. Only prohemocytes and plasmatocytes can be clearly classified; all the other hemocyte types have a more ambiguous classification.
通过相差显微镜和普通光学显微镜,利用一些组织化学方法,在大劣按蚊的血淋巴中鉴定出了五种血细胞类型。它们分别是:原血细胞,细胞核与细胞质比例很大的小细胞;浆血细胞,数量最多的血细胞,是多形细胞,主要特征是含有大量溶酶体;粒细胞,与浆血细胞非常相似的血细胞,含有细胞质颗粒,尤其富含多糖;oenocytoids细胞,细胞核小且细胞质厚;在吉姆萨涂片观察时可见许多小圆形液泡,在相差显微镜下可见许多细胞质颗粒;脂肪血细胞,非常大的血细胞,有许多脂肪滴包涵体,可能对应进入血淋巴的游离脂肪体。只有原血细胞和浆血细胞能够被明确分类;所有其他血细胞类型的分类则更为模糊。