使用重组杆状病毒进行单纯疱疹病毒1型（HSV-1）蛋白表达。
HSV-1 protein expression using recombinant baculoviruses.
作者信息
Grady Lorry M, Bai Ping, Weller Sandra K
机构信息
Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT, 06030-3205, USA.
出版信息
Methods Mol Biol. 2014;1144:293-304. doi: 10.1007/978-1-4939-0428-0_20.
The baculovirus expression system is an invaluable method for the expression of Herpes Simplex Virus 1 (HSV-1) proteins. The use of insect cells provides a eukaryotic system for the robust expression of heterologous proteins under control of the baculovirus polyhedrin gene promoter that naturally drives the high expression of the polyhedrin protein. Additionally, insect cells often initiate the necessary posttranslational modifications and/or disulfide-bond formation important for the proper folding of the protein. We and others have successfully expressed and purified several HSV-1 proteins including the polymerase, helicase-primase, single-strand DNA binding protein, and alkaline nuclease. The following protocol is based on 15 years of experience from our laboratory.
杆状病毒表达系统是表达单纯疱疹病毒1型（HSV-1）蛋白的一种非常有价值的方法。使用昆虫细胞提供了一个真核系统，可在杆状病毒多角体蛋白基因启动子的控制下强劲表达异源蛋白，该启动子天然驱动多角体蛋白的高表达。此外，昆虫细胞通常会启动对蛋白质正确折叠很重要的必要翻译后修饰和/或二硫键形成。我们和其他人已经成功表达并纯化了几种HSV-1蛋白，包括聚合酶、解旋酶-引发酶、单链DNA结合蛋白和碱性核酸酶。以下方案基于我们实验室15年的经验。
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