Carbajal Daisy, Arruzazabala Maria de Lourdes, Rosa Más, Molina Vivian, Rodríguez Eduardo, González Victor
Centre of Natural Products, National Center for Scientific Research, Havana, Cuba.
Curr Ther Res Clin Exp. 2004 Nov;65(6):505-14. doi: 10.1016/j.curtheres.2005.01.001.
Benign prostatic hypertrophy is the nonmalignant, uncontrolled growth of prostatic epithelial cells and stroma that, left untreated, may lead to difficult urination and other complications. A common treatment of BPH is lipid extract from saw palmetto fruit, and lipid extract from Cuban Royal palm (a palm of the same family) fruit is being studied for this use. One study found that the latter, D-004, at 100 to 400 mg/kg daily prevented prostatic hypertrophy (PH) induced with testosterone (T) in a rat model.
This study comprised 2 experiments in a rat model. The first assessed the effects of different doses of D-004 on T-induced PH; the second investigated the effects of D-004 on PH induced with dihydrotestosterone (DHT).
In experiment 1, rats were distributed in 6 groups of 10 rats each. One group received an SC injection of soy oil and oral treatment with Tween 65/water vehicle (negative control). The other 5 groups received an SC injection of T 3 mg/kg daily and oral treatment with vehicle (positive control) or D-004 at 50, 200, 400, or 800 mg/kg daily suspended in vehicle. In experiment 2, rats were distributed in 3 groups of 10 rats each. A negative control group received treatment as in experiment 1. Positive controls received an SC injection of DHT 1.5 mg/kg and vehicle orally. The third group received an SC injection of DHT and oral treatment with D-004 at 800 mg/kg suspended in vehicle. All treatments were given for 14 days. At sacrifice, prostates were removed and weighed. Mean prostatic weights and prostatic/body weight ratios were calculated.
In experiment 1, in the groups receiving D-004 at 200, 400, or 800 mg/kg daily, prostatic weight was significantly lower compared with the positive control group (P < 0.05, P < 0.01, and P < 0.001, respectively); this effect was not seen in the group receiving 50 mg/kg daily. In the groups receiving D-004 at 400 and 800 mg/kg daily, prostatic/body weight ratio was significantly lower compared with positive controls (both, P < 0.05); this effect was not seen in the groups receiving 50 or 200 mg/kg daily. In experiment 2, prostatic weight and prostatic/body weight ratio in the group receiving D-004 were similar to those of positive controls. Body weight was not affected in any of the groups receiving D-004.
This study of rats with T- or DHT-induced PH suggests that D-004 at 200 to 800 mg/kg daily administered orally prevents T-induced PH, and that D-004 at 800 mg/kg daily does not prevent DHT-induced PH.
良性前列腺增生是前列腺上皮细胞和基质的非恶性、不受控制的生长，若不治疗，可能导致排尿困难及其他并发症。良性前列腺增生的一种常见治疗方法是使用锯棕榈果实的脂质提取物，古巴皇家棕榈（同一家族的棕榈树）果实的脂质提取物也正为此用途而进行研究。一项研究发现，后者（D - 004），每日剂量为100至400毫克/千克，可预防大鼠模型中由睾酮（T）诱导的前列腺增生（PH）。
本研究在大鼠模型中进行了2个实验。第一个实验评估不同剂量的D - 004对T诱导的前列腺增生的影响；第二个实验研究D - 004对二氢睾酮（DHT）诱导的前列腺增生的影响。
在实验1中，将大鼠分成6组，每组10只。一组接受皮下注射大豆油并口服吐温65/水载体（阴性对照）。其他5组每日皮下注射3毫克/千克的T，并口服载体（阳性对照）或每日剂量为50、200、400或800毫克/千克悬浮于载体中的D - 004。在实验2中，将大鼠分成3组，每组10只。阴性对照组的处理与实验1相同。阳性对照组接受皮下注射1.5毫克/千克的DHT并口服载体。第三组接受皮下注射DHT并口服每日剂量为800毫克/千克悬浮于载体中的D - 004。所有处理均持续14天。处死大鼠时，取出前列腺并称重。计算平均前列腺重量及前列腺/体重比值。
在实验1中，每日接受200、400或800毫克/千克D - 004的组，其前列腺重量与阳性对照组相比显著降低（分别为P < 0.05、P < 0.01和P < 0.001）；每日接受50毫克/千克的组未观察到这种效果。每日接受400和800毫克/千克D - 004的组，其前列腺/体重比值与阳性对照组相比显著降低（均为P < 0.05）；每日接受50或200毫克/千克的组未观察到这种效果。在实验2中，接受D - 004的组的前列腺重量和前列腺/体重比值与阳性对照组相似。接受D - 004的任何组的体重均未受影响。
这项对T或DHT诱导的前列腺增生大鼠的研究表明，每日口服200至800毫克/千克的D - 004可预防T诱导的前列腺增生，而每日800毫克/千克的D - 004不能预防DHT诱导的前列腺增生。
