基于聚合酶链反应和脱氧核糖核酸测序的人乳头瘤病毒16/18在宫颈上皮内瘤变组织病理学类型及原发性浸润性宫颈癌中的分布研究：印度北孟加拉邦的情况

Polymerase chain reaction and deoxyribonucleic acid-sequencing based study on distribution of human papillomavirus 16/18 among histopathological types of cervical intra-epithelial neoplasia and primary invasive cervical carcinoma: A scenario in North Bengal, India.

作者信息

Ghosh Prithwijit, Ghosh Damayanti Das, Majumdar Giri Amita, Sengupta Sharmila, Das Chandana, Mukhopadhyay Indranil

机构信息

Department of Pathology, North Bengal Medical College and Hospital, Siliguri, India ; Department of Pathology, DESUN (NEON) Lab, Kolkata, West Bengal, India.

Human Genetics Unit, Biological Sciences Division, Indian Statistical Institute, Kolkata, West Bengal, India ; Cancer Biology and Inflammatory Disorder Division, Indian Institute of Chemical Biology, Kolkata, West Bengal, India.

出版信息

J Midlife Health. 2014 Jan;5(1):14-22. doi: 10.4103/0976-7800.127786.

DOI:10.4103/0976-7800.127786

PMID:24672201

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3955040/

Abstract

INTRODUCTION

Human papillomavirus (HPV) types 16/18 are reportedly most common in cervical cancer (CaCx) with geographical variation of genotypes. HPV16 predominates both in squamous cell carcinoma (SCC) and adenocarcinoma in India, contrary to reported global predominance of HPV18 in the latter. Our study was aimed to determine the occurrence of HPV16/18 among histopathological types of cervical intra-epithelial neoplasia (CIN) and invasive CaCx from North Bengal, India and to identify any major deviation from the known Indian scenario of distribution of HPV16/18 genotypes in cases of SCC and adenocarcinoma.

MATERIALS AND METHODS

This was a retrospective, cross-sectional, case-only type of study, in which 40 cases were histopathologically diagnosed as CIN/CaCx, on which polymerase chain reaction (PCR), deoxyribonucleic acid (DNA)-sequencing and bioinformatics by basic search local alignment tool were performed for HPV-genotyping.

STATISTICAL ANALYSIS

The distribution of HPV genotypes among cases of SCC and adenocarcinoma was compared by Fisher's exact-test.

RESULTS

HPV was detected in 97.5% (39/40) cases. HPV16-infected cases (32/39; 82.05%) predominated over HPV18-infected ones (7/39; 17.95%). However, HPV18-only infection was significantly (P = 0.0045, one-sided Fisher's exact test) more among adenocarcinoma (3/4; 75%) than SCC (2/26; 7.69%) contrary to HPV16-only infection (SCC = 24/26, 92.31%; adenocarcinoma = 1/4; 25%) whereas both CIN3 cases were HPV16-positive.

CONCLUSION

Predominance of HPV18 over HPV16 in cases of adenocarcinoma in this region was contrasting to that of earlier Indian studies suggesting research on HPV18 related cervical carcinogenesis. PCR and DNA-sequencing could prove to be highly effective tools in HPV detection and genotyping. The study reported HPV16/18 infection in almost 98% of the cases, the knowledge about which might prove useful in future population based studies on HPV genotyping and designing of appropriate HPV-vaccines for this region.

摘要

引言

据报道，16/18型人乳头瘤病毒（HPV）在宫颈癌（CaCx）中最为常见，且基因型存在地域差异。在印度，HPV16在鳞状细胞癌（SCC）和腺癌中均占主导地位，这与全球报道的后者中HPV18占主导地位的情况相反。我们的研究旨在确定印度北孟加拉地区宫颈上皮内瘤变（CIN）和浸润性CaCx的组织病理学类型中HPV16/18的发生率，并确定在SCC和腺癌病例中HPV16/18基因型分布的已知印度情况是否存在任何重大偏差。

材料与方法

这是一项回顾性、横断面、仅病例类型的研究，其中40例经组织病理学诊断为CIN/CaCx，对其进行聚合酶链反应（PCR）、脱氧核糖核酸（DNA）测序，并通过基本搜索局部比对工具进行生物信息学分析以进行HPV基因分型。

统计分析

通过Fisher精确检验比较SCC和腺癌病例中HPV基因型的分布。

结果

97.5%（39/40）的病例检测到HPV。HPV16感染病例（32/39；82.05%）多于HPV18感染病例（7/39；17.95%）。然而，仅HPV18感染在腺癌（3/4；75%）中显著（P = 0.0045，单侧Fisher精确检验）多于SCC（2/26；7.69%），这与仅HPV16感染情况相反（SCC = 24/26，92.31%；腺癌 = 1/4；25%），而两例CIN3病例均为HPV16阳性。

结论

该地区腺癌病例中HPV18比HPV16占主导地位，这与早期印度研究结果形成对比，提示对HPV18相关宫颈癌发生机制进行研究。PCR和DNA测序可能是HPV检测和基因分型的高效工具。该研究报告几乎98%的病例存在HPV16/18感染，这一知识可能对未来基于人群的HPV基因分型研究以及为该地区设计合适的HPV疫苗有用。