Fan Run-Zhen, Liu Congyun, Jiang Wenqing, Wang Xiaonan, Liu Fengmao
J AOAC Int. 2014 Jan-Feb;97(1):183-7. doi: 10.5740/jaoacint.12-194.
Ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) based on solidification of the floating organic solvent droplets (SFO) combined with HPLC was used for determination of five fungicides in fruit juice samples. 1-Dodecanol, which has a low density and low toxicity, was used as the extraction solvent in UA-DLLME. The solidification of floating organic droplets facilitates the transfer of analytes from the aqueous phase to the organic phase. This method was easy, quick, inexpensive, precise, and linear over a wide range. Under the optimized conditions, the enrichment factors for a 5 mL fruit juice sample were 25 to 56, and the LODs for the five fungicides ranged from 5 to 50 microg/L. The average recoveries ranged from 71.8 to 118.2% with RSDs of 0.9 to 13.9%. Application of the DLLME-SFO technique allows successful separation and preconcentration of the fungicides at a low concentration level in fruit juice samples.
基于浮动有机溶剂滴固化（SFO）的超声辅助分散液液微萃取（UA-DLLME）结合高效液相色谱法用于测定果汁样品中的五种杀菌剂。密度低且毒性低的1-十二醇被用作UA-DLLME中的萃取溶剂。浮动有机滴的固化促进了分析物从水相转移到有机相。该方法简便、快速、廉价、精确，且在很宽的范围内呈线性。在优化条件下，5 mL果汁样品的富集因子为25至56，五种杀菌剂的检出限为5至50 μg/L。平均回收率为71.8%至118.2%，相对标准偏差为0.9%至13.9%。DLLME-SFO技术的应用能够成功地在低浓度水平下对果汁样品中的杀菌剂进行分离和预富集。
