Tran Buu N, Okoniewski Richard, Bucciferro Anthony, Jansing Robert, Aldous Kenneth M
J AOAC Int. 2014 Jan-Feb;97(1):232-7. doi: 10.5740/jaoacint.12-198.
Contamination of drinking water by ethylene glycol (EG) is a public health concern. EG causes adverse health effects in humans and animals, including cardiopulmonary and acute renal failure. EG and other glycols, such as propylene glycol (PG) are major components in antifreeze liquids, which may be the main source of contamination of ground water. A sensitive LC/electrospray ionization (ESI)-MS/MS method was developed to measure trace amounts of EG, diethylene glycol, and 1,2- and 1,3-PG in several water sources, including municipal tap, lake, river, and salinated water. In this method, glycols in water samples were derivatized with benzoyl chloride by the Schotten-Baumann reaction, followed by liquid-liquid extraction using pentane as the organic solvent prior to the LC/ESI-MS/MS determination. QC included analysis of a method blank and samples fortified at low and high levels. Analytical data showed excellent linear calibration for all observed glycols, with good precision and accuracy. The method detection limits for the studied glycols ranged from 1.9 to 6.1 ng/mL across the water matrixes tested. This method is suitable to help assess water quality in areas that may be prone to glycol contamination.
乙二醇（EG）对饮用水的污染是一个公共卫生问题。EG会对人类和动物产生不良健康影响，包括心肺功能衰竭和急性肾衰竭。EG以及其他二醇类物质，如丙二醇（PG），是防冻液中的主要成分，而防冻液可能是地下水污染的主要来源。本文开发了一种灵敏的液相色谱/电喷雾电离（ESI）-串联质谱法，用于测定多种水源（包括市政自来水、湖水、河水和盐水）中的痕量EG、二甘醇以及1,2-丙二醇和1,3-丙二醇。在该方法中，水样中的二醇类物质通过肖滕-鲍曼反应与苯甲酰氯衍生化，然后在进行LC/ESI-MS/MS测定之前，以戊烷作为有机溶剂进行液-液萃取。质量控制包括分析方法空白以及低浓度和高浓度加标样品。分析数据表明，所有观察到的二醇类物质均具有出色的线性校准，精密度和准确度良好。在所测试的各种水基质中，所研究二醇类物质的方法检出限范围为1.9至6.1 ng/mL。该方法适用于帮助评估可能容易受到二醇污染地区的水质。
